Leite Renata Sousa, da Rocha Rogério Gonçalves, Lima Tabosa Angeliny Tamiarana, Batista Gomes Emisael Stênio, Santiago Laís, Rodrigues Danillo Costa, Sousa Santos Sérgio Henrique, Sena Guimarães André Luiz, Farias Lucyana Conceição
Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Minas Gerais, Brazil.
Oncological Radiology Center, Hospital Dilson Godinho, Montes Claros, Minas Gerais, Brazil.
Int J Mol Cell Med. 2024;13(1):19-28. doi: 10.22088/IJMCM.BUMS.13.1.19.
Gallic acid (GA) is a powerful antioxidant extracted from plants of the Brazilian Cerrado. Oxidative stress plays an important role in the occurrence of radiation-induced osteonecrosis in patients treated for head and neck cancer. There is a need to develop research aimed at developing complementary therapies to prevent or reverse bone damage. The aim of the present study was to investigate the effect of GA in preosteoblasts exposed to therapeutic ionizing radiation. MC3T3-E1 preosteoblast cells were treated with 10 µM GA and exposed to 6 Gy ionizing radiation. We performed assays of cell proliferation, oxidative stress analysis by detection of reactive oxygen species, and alkaline phosphatase assay. GA at lower concentrations was able to significantly increase proliferation and inhibit radiation-induced generation of reactive oxygen species in osteoblast precursor cells, despite ionizing radiation-induced injury. Furthermore, GA significantly increased alkaline phosphatase at a dose of 6 Gy. The findings suggested that GA could attenuate ionizing radiation-induced injuries in osteoblast precursor cells. Moreover, studies are needed to better investigate the role of GA in osteonecrosis, especially in cancer patients undergoing radiotherapy or taking antiresorptive drugs.
没食子酸（GA）是一种从巴西塞拉多植物中提取的强效抗氧化剂。氧化应激在接受头颈癌治疗的患者发生放射性骨坏死中起重要作用。有必要开展旨在开发补充疗法以预防或逆转骨损伤的研究。本研究的目的是调查GA对暴露于治疗性电离辐射的前成骨细胞的影响。将MC3T3-E1前成骨细胞用10 µM GA处理并暴露于6 Gy电离辐射。我们进行了细胞增殖测定、通过检测活性氧进行氧化应激分析以及碱性磷酸酶测定。尽管存在电离辐射诱导的损伤，但较低浓度的GA能够显著增加成骨细胞前体细胞的增殖并抑制辐射诱导的活性氧生成。此外，GA在6 Gy剂量下显著增加了碱性磷酸酶。这些发现表明GA可以减轻电离辐射对成骨细胞前体细胞的损伤。此外，需要进行更多研究以更好地调查GA在骨坏死中的作用，特别是在接受放疗或服用抗吸收药物的癌症患者中。
