系统性红斑狼疮患者中P105阴性B细胞亚群的表型分析。
Phenotyping of P105-negative B cell subsets in patients with systemic lupus erythematosus.
作者信息
Koarada Syuichi, Tada Yoshifumi, Suematsu Rie, Soejima Sachiko, Inoue Hisako, Ohta Akihide, Nagasawa Kohei
机构信息
Division of Rheumatology, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga 849-8501, Japan.
出版信息
Clin Dev Immunol. 2012;2012:198206. doi: 10.1155/2012/198206. Epub 2011 Sep 26.
This study aimed to investigate phenotype of RP105(-) B cell subsets in patients with systemic lupus erythematosus (SLE). Flow cytometry was used for phenotyping RP105-negaive B cell subsets. Based on CD19, RP105, and CD138 expression, RP105(-) B cells consist of at least 5 subsets of late B cells, including CD19(+)RP105(int), CD19(+) RP105(-), CD19(low) RP105(-) CD138(-), CD19(low) RP105(-)CD138(int), and CD19(low) RP105(-) CD138(++) B cells. Especially, CD19(+)RP105(int) and CD19(low) RP105(-)CD138(int) B cells are significantly larger than other RP105(-) B cell subsets in SLE. By comparison of RP105(-) B cell subsets between patients with SLE and normal subjects, these subsets were detectable even in normal subjects, but the percentages of RP105(-) B cell subsets were significantly larger in SLE. The phenotypic analysis of RP105(-) B cell subsets suggests dysregulation of later B cell subsets in SLE and may provide new insights into understanding regulation of B cells in human SLE.
本研究旨在调查系统性红斑狼疮（SLE）患者中RP105阴性B细胞亚群的表型。采用流式细胞术对RP105阴性B细胞亚群进行表型分析。基于CD19、RP105和CD138的表达，RP105阴性B细胞至少由5个晚期B细胞亚群组成，包括CD19(+)RP105(int)、CD19(+)RP105(-)、CD19(low)RP105(-)CD138(-)、CD19(low)RP105(-)CD138(int)和CD19(low)RP105(-)CD138(++)B细胞。特别是，在SLE中，CD19(+)RP105(int)和CD19(low)RP105(-)CD138(int)B细胞明显大于其他RP105阴性B细胞亚群。通过比较SLE患者和正常受试者的RP105阴性B细胞亚群，发现这些亚群在正常受试者中也可检测到，但SLE中RP105阴性B细胞亚群的百分比明显更高。RP105阴性B细胞亚群的表型分析表明SLE中晚期B细胞亚群存在失调，可能为理解人类SLE中B细胞的调节提供新的见解。
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