Barroso-Villa Gerardo, Colin-Valenzuela Alinne, Gómez-López Nardhy, Velázquez-Magaña Mauricio
Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes.
Ginecol Obstet Mex. 2010 Dec;78(12):685-91.
The packaging of heterochromatin during spermatogenesis has been correlated with the expression of residual apoptotic bodies (which stain with merocyanine A) that will impact on sperm function in the fertilization process; as well as the joint expression of the transmembrane translocation phosphatidyl serine and oligonucleosomes.
To evaluate the expression of bodies stained with merocyanine in the functional processes of sperm and their level of agreement with apoptotic Annexin V and TUNEL biomarkers.
We performed a prospective, cross, including 11,000 cells belonging to semen samples from infertile men, were evaluated according to WHO criteria (1999), bounded by the lines of Tygerberg. The biomolecular transformation processing of the membrane and the expression of oligonucleosomes in the terminal cascade of apoptosis were quantified by cytometry flow, using an argon lasser as a reading source of 480 nm, discriminating the degree of cellularity, both negative and positive for each indicators.
Because of the study design was found low quantification in semen parameters, motility, morphology and sperm concentration. The average expression of cells [DNA-PI(+) / dUTP-FITC(+)] (quantification of TUNEL) and [Annexin-V(+) / PI(-)] was 36.5 +/- 17.4% and 31.2 +/- 17.4%, respectively. By comparing the expression of TUNEL without the effect of M540 bodies (36.3 +/- 1.7% vs. 36 +/- 1.7%) a significant difference was not determined.
This study shows that there is a remnant of the primary processes of spermiation, which can take an important role in apoptotic and functional processes of the sperm. However, its expression does not affect measurement of biomarkers of apoptosis seminal, whose determination changed the diagnosis and functional perception of reproductive parameters in the sperm.
精子发生过程中异染色质的包装与残留凋亡小体（用部花青A染色）的表达相关，这会影响受精过程中的精子功能；还与跨膜易位磷脂酰丝氨酸和寡核小体的联合表达有关。
评估部花青染色小体在精子功能过程中的表达及其与凋亡膜联蛋白V和TUNEL生物标志物的一致性水平。
我们进行了一项前瞻性交叉研究，纳入了11000个来自不育男性精液样本的细胞，根据世界卫生组织（1999年）标准并以泰格堡线为界进行评估。使用氩激光作为480nm的读取源，通过流式细胞术对凋亡终末级联反应中膜的生物分子转化过程和寡核小体的表达进行定量，区分每个指标的阴性和阳性细胞程度。
由于研究设计，精液参数、活力、形态和精子浓度的定量较低。细胞[DNA-PI(+) / dUTP-FITC(+)]（TUNEL定量）和[膜联蛋白-V(+) / PI(-)]的平均表达分别为36.5 +/- 17.4%和31.2 +/- 17.4%。比较无M540小体影响时的TUNEL表达（36.3 +/- 1.7%对36 +/- 1.7%），未确定有显著差异。
本研究表明，精子形成的初级过程存在残余物，其在精子的凋亡和功能过程中可能起重要作用。然而，其表达并不影响精液凋亡生物标志物的测量，这些生物标志物的测定改变了对精子生殖参数的诊断和功能认知。
