多不饱和脂肪酸处理的神经胶质瘤细胞的 microRNA 谱揭示了凋亡特异性的表达变化。
MicroRNA profile of polyunsaturated fatty acid treated glioma cells reveal apoptosis-specific expression changes.
机构信息
Functional Genomics Laboratory, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary.
出版信息
Lipids Health Dis. 2011 Sep 30;10:173. doi: 10.1186/1476-511X-10-173.
Abstract
BACKGROUND
Polyunsaturated fatty acids (PUFAs) such as γ-linolenic acid (GLA), arachidonic acid (AA) and docosahexaenoic acid (DHA) have cytotoxic action on glioma cells.
RESULTS
We evaluated the cytotoxic action of GLA, AA and DHA on glioma cells with specific reference to the expression of miRNAs. Relative expression of miRNAs were assessed by using high throughput nanocapillary real-time PCR. Most of the miRNA target genes that showed altered expression could be classified as apoptotic genes and were up-regulated by PUFA or temozolomide treatment, while similar treatments resulted in repression of the corresponding mRNAs, such as cox2, irs1, irs2, ccnd1, itgb3, bcl2, sirt1, tp53inp1 and k-ras.
CONCLUSIONS
Our results highlight involvement of miRNAs in the induction of apoptosis in glioma cells by fatty acids and temozolomide.
摘要
背景
多不饱和脂肪酸（PUFAs）如γ-亚麻酸（GLA）、花生四烯酸（AA）和二十二碳六烯酸（DHA）对神经胶质瘤细胞具有细胞毒性作用。
结果
我们评估了 GLA、AA 和 DHA 对神经胶质瘤细胞的细胞毒性作用，并特别参考了 miRNA 的表达。使用高通量纳米毛细管实时 PCR 评估 miRNA 的相对表达。大多数表现出改变表达的 miRNA 靶基因可归类为凋亡基因，并被 PUFAs 或替莫唑胺处理上调，而类似的处理导致相应的 mRNAs 被抑制，如 cox2、irs1、irs2、ccnd1、itgb3、bcl2、sirt1、tp53inp1 和 k-ras。
结论
我们的结果强调了 miRNA 参与了脂肪酸和替莫唑胺诱导神经胶质瘤细胞凋亡的过程。
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