Xu X F, Björntorp P
Wallenberg Laboratory, Sahlgren's Hospital, University of Göteborg, Sweden.
Exp Cell Res. 1990 Aug;189(2):247-52. doi: 10.1016/0014-4827(90)90243-4.
The effects of dexamethasone (DEX) on adipose precursor cells from rat adipose tissue were studied in primary culture. When added from the beginning of culture in media containing untreated fetal calf serum (SM), serum treated with charcoal to remove steroid hormones (CSM), or serum-free medium (SFM), DEX inhibited cellular growth. Lipoprotein lipase (LPL) as well as glycerophosphate dehydrogenase (GPDH) activities, markers of cellular differentiation, were also inhibited, except in CSM where LPL was stimulated. When added after cellular confluence, however, DEX had opposite effects and now stimulated cellular differentiation. This effect was highly dependent on insulin. These studies demonstrate that DEX affects adipose precursor cells in several ways, depending on the type of culture medium, the time period of exposure, and the presence of insulin.
研究了地塞米松（DEX）对原代培养的大鼠脂肪组织脂肪前体细胞的影响。当在含有未处理胎牛血清（SM）、经活性炭处理以去除类固醇激素的血清（CSM）或无血清培养基（SFM）的培养基中从培养开始就添加DEX时，DEX会抑制细胞生长。细胞分化标志物脂蛋白脂肪酶（LPL）以及甘油磷酸脱氢酶（GPDH）的活性也受到抑制，但在CSM中LPL受到刺激。然而，当在细胞汇合后添加DEX时，DEX会产生相反的效果，此时会刺激细胞分化。这种效应高度依赖于胰岛素。这些研究表明，DEX通过多种方式影响脂肪前体细胞，这取决于培养基的类型、暴露时间段以及胰岛素的存在。
