Xu X F, Björntorp P
Department of Medicine I, Sahlgren's Hospital, Göteborg, Sweden.
Exp Cell Res. 1987 Dec;173(2):311-21. doi: 10.1016/0014-4827(87)90273-4.
The effects of estradiol-17 beta and progesterone on multiplication, differentiation and lipid filling of adipose precursor cells were examined in primary cell cultures of cells prepared from adipose tissue of both male and ovariectomized female rats. Progesterone down to a concentration of 10(-7) mol/liter, alone or in the presence of estradiol-17 beta stimulated the development of glycerophosphate dehydrogenase and lipoprotein lipase activity. Estradiol-17 beta alone had no effects. These effects were essentially parallel to increases in the rate of lipid filling of the cells. Furthermore, the formation of cells with a lipid vacuole greater than 20 micron was markedly stimulated, suggesting that new fat cells were formed by the stimulation of differentiation of the adipose precursor cells. No effects of the sex steroid hormones were seen on the rate of multiplication. These results suggest a role of sex steroid hormones in the regulation of triglyceride storage capacity in adipose tissue by facilitating the differentiation of precursor cells to form new adipocytes.
在从雄性大鼠和去卵巢雌性大鼠脂肪组织制备的原代细胞培养物中，研究了17β-雌二醇和孕酮对脂肪前体细胞增殖、分化和脂质填充的影响。单独或在17β-雌二醇存在的情况下，低至10^(-7)摩尔/升浓度的孕酮刺激了磷酸甘油脱氢酶和脂蛋白脂肪酶活性的发展。单独的17β-雌二醇没有作用。这些作用基本上与细胞脂质填充率的增加平行。此外，脂质空泡大于20微米的细胞形成受到明显刺激，这表明通过刺激脂肪前体细胞的分化形成了新的脂肪细胞。未观察到性类固醇激素对增殖速率有影响。这些结果表明，性类固醇激素通过促进前体细胞分化形成新的脂肪细胞，在调节脂肪组织中甘油三酯储存能力方面发挥作用。
