College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China.
Ecotoxicol Environ Saf. 2012 Feb;76(2):175-81. doi: 10.1016/j.ecoenv.2011.09.009. Epub 2011 Oct 1.
Adult Eisenia fetida earthworms were exposed to sub-lethal concentrations of 1,2,4-trichlorobenzene to assess the toxicity of contaminated soils. The LC₅₀ of 1,2,4-TCB at 7 and 14 d were 945±175 and 890±169 mg kg⁻¹. A lower dose of 1,2,4-TCB (25 mg g⁻¹) had stimulatory effects on SOD and AChE activities, but AChE activities were significantly inhibited at higher treatment levels (100 and 400 mg g⁻¹). SOD activities increased after 2, 7, and 14 d of exposure, but AChE activities were significantly inhibited at 400 mg/kg 1,2,4-TCB after 2, 7, and 14 d of exposure, and the inhibition rates were 25.41%, 28.65%, and 25.05%. Ultrastructural observation of the intestinal epithelium at three concentrations (control, 50, and 400 mg g⁻¹) revealed that the epicuticle, cuticle layer, and microvilli were damaged with increasing 1,2,4-TCB concentration. At 400 mg kg⁻¹, mitochondria were seriously injured and the smooth endoplasmic reticulum was seriously dilated.
成年赤子爱胜蚓暴露于亚致死浓度的 1,2,4-三氯苯以评估污染土壤的毒性。在第 7 和 14 天，1,2,4-TCB 的 LC₅₀ 值分别为 945±175 和 890±169 mg kg⁻¹。较低剂量的 1,2,4-TCB（25 mg g⁻¹）对 SOD 和 AChE 活性有刺激作用，但在较高处理水平（100 和 400 mg g⁻¹）时 AChE 活性显著受到抑制。SOD 活性在暴露后 2、7 和 14 天增加，但在暴露后 2、7 和 14 天，400 mg/kg 1,2,4-TCB 对 AChE 活性的抑制率分别为 25.41%、28.65%和 25.05%。在三个浓度（对照、50 和 400 mg g⁻¹）下对肠上皮的超微结构观察表明，随着 1,2,4-TCB 浓度的增加，表皮层、角质层和微绒毛受损。在 400 mg kg⁻¹时，线粒体受到严重损伤，光滑内质网严重扩张。
