Australian Centre for Research on Separation Science, School of Natural Sciences, University of Western Sydney (Parramatta), Sydney, NSW, Australia.
J Chromatogr A. 2012 Apr 6;1232:47-51. doi: 10.1016/j.chroma.2011.09.021. Epub 2011 Sep 14.
A new column technology - termed parallel segmented outlet flow was employed here to illustrate gains in separation performance that are achievable by the active management of flow as it exits from the outlet of the chromatography column. Parallel segmented outlet flow requires a column be fitted with an outlet fitting that separates flow from the central region of the column from that of wall region. Each region of flow is able to be processed independently, such that post column detection emulates end column localised detection. As a result of this flow segmentation and the subsequent more efficient means of detection, column efficiency was observed to increase by more than 20%, with gains in sensitivity by as much as 22%, and a decrease in peak volume by up to 85%.
一种新的柱技术 - 称为并行分段出口流，在这里被采用，以说明通过主动管理流动从色谱柱出口流出时可以实现的分离性能的提高。并行分段出口流需要在柱上安装一个出口配件，该配件将柱的中心区域与壁区域的流动分开。每个流动区域都能够独立处理，使得柱后检测模拟柱端局部检测。由于这种流动分段和随后更有效的检测手段，观察到柱效率增加了超过 20%，灵敏度提高了高达 22%，峰体积减少了高达 85%。
