Pravadali-Cekic Sercan, Kocic Danijela, Hua Stanley, Jones Andrew, Dennis Gary R, Shalliker R Andrew
School of Science and Health, University of Western Sydney.
School of Science and Health, University of Western Sydney;
J Vis Exp. 2015 Dec 15(106):e53448. doi: 10.3791/53448.
Active flow technology (AFT) is new form of column technology that was designed to overcome flow heterogeneity to increase separation performance in terms of efficiency and sensitivity and to enable multiplexed detection. This form of AFT uses a parallel segmented flow (PSF) column. A PSF column outlet end-fitting consists of 2 or 4 ports, which can be multiplexed to connect up to 4 detectors. The PSF column not only allows a platform for multiplexed detection but also the combination of both destructive and non-destructive detectors, without additional dead volume tubing, simultaneously. The amount of flow through each port can also be adjusted through pressure management to suit the requirements of a specific detector(s). To achieve multiplexed detection using a PSF column there are a number of parameters which can be controlled to ensure optimal separation performance and quality of results; that is tube dimensions for each port, choice of port for each type of detector and flow adjustment. This protocol is intended to show how to use and tune a PSF column functioning in a multiplexed mode of detection.
主动流动技术（AFT）是一种新型的柱技术，旨在克服流动不均一性，以提高分离效率和灵敏度，并实现多重检测。这种形式的AFT使用平行分段流（PSF）柱。PSF柱的出口端配件由2个或4个端口组成，这些端口可以多路复用，最多可连接4个检测器。PSF柱不仅为多重检测提供了一个平台，而且还能同时组合使用破坏性和非破坏性检测器，无需额外的死体积管路。每个端口的流量也可以通过压力管理进行调整，以适应特定检测器的要求。要使用PSF柱实现多重检测，可以控制多个参数以确保最佳的分离性能和结果质量；这些参数包括每个端口的管径、每种类型检测器的端口选择以及流量调整。本方案旨在展示如何使用和调整以多重检测模式运行的PSF柱。
