College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
Talanta. 2011 Oct 15;85(5):2301-6. doi: 10.1016/j.talanta.2011.04.012. Epub 2011 Apr 12.
Comparison of hydrophilic interaction chromatography (HILIC) columns coupled with an evaporative light scattering detector (ELSD) or charged aerosol detector (CAD) was done for the detection of gabapentin in pharmaceutical formulations. The chromatographic separations were achieved on four HILIC columns: ZIC HILIC, ZIC pHILIC, Luna HILIC, and Atlantis HILIC. Experimental factors such as mobile phase composition, acetonitrile content, and mobile phase pH were evaluated. Validation of method was done in terms of linearity, sensitivity, accuracy, and precision. The performance of ELSD detection method is comparable to that of CAD. The intra-day and inter-day variations were below 1.7% and 3.2% for CAD and 2.8%, and 3.4% for ELSD, respectively. In addition, detection sensitivities of ELSD, CAD, and UV detectors were also compared for HILIC and reversed phase (RP) modes and the highest sensitivities were obtained in the HILIC mode when connected with CAD and ELSD. The developed HILIC aerosol based detection methods were successfully applied to the analysis of gabapentin in commercial tablets and capsules.
对亲水作用色谱（HILIC）柱与蒸发光散射检测器（ELSD）或带电气溶胶检测器（CAD）进行了比较，用于检测药物制剂中的加巴喷丁。在四种 HILIC 柱上实现了色谱分离：ZIC HILIC、ZIC pHILIC、Luna HILIC 和 Atlantis HILIC。评估了流动相组成、乙腈含量和流动相 pH 等实验因素。该方法通过线性、灵敏度、准确度和精密度进行了验证。ELSD 检测方法的性能可与 CAD 相媲美。CAD 和 ELSD 的日内和日间变异分别低于 1.7%和 3.2%和 2.8%和 3.4%。此外，还比较了 ELSD、CAD 和 UV 检测器在 HILIC 和反相（RP）模式下的检测灵敏度，当与 CAD 和 ELSD 连接时，在 HILIC 模式下获得了最高的灵敏度。开发的基于 HILIC 气溶胶的检测方法成功应用于商业片剂和胶囊中加巴喷丁的分析。
