College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
J Pharm Biomed Anal. 2010 Mar 11;51(4):973-8. doi: 10.1016/j.jpba.2009.10.019. Epub 2009 Oct 31.
Recently, charged aerosol detection (CAD), a new kind of universal detection method, has been widely employed in the HPLC system. In the present study, four kinds of anti-diabetic drug standards, glipizide, gliclazide, glibenclamide and glimepiride were determined by ultraviolet (UV) detection, evaporative light scattering detection (ELSD) and the aforementioned CAD. The results were compared with reference to linearity, accuracy, precision and limit of detection (LOD). All of the experiments were performed on a reverse phase column with water and acetonitrile as the mobile phase. Separations were achieved under the same chromatographic conditions for each detection method. As a result, CAD generated nearly uniform responses compared with UV detection and ELSD. It showed the best accuracy and LOD among 3 detectors and had similar precision with UV detection at higher concentrations while UV detection showed a better precision at lower concentrations than did CAD or ELSD. The LOD of CAD, in fact, can be up to two times higher than that of ELSD. The UV and ELSD linearity was satisfactory at R(2)>0.99, though in the case of CAD, a log-log transformation was needed. The proposed methods were also applied to the real anti-diabetic drugs and diabetes-related dietary supplements.
最近，荷电气溶胶检测（CAD）作为一种新的通用检测方法已在 HPLC 系统中得到广泛应用。本研究采用紫外（UV）检测、蒸发光散射检测（ELSD）和上述 CAD 对四种抗糖尿病药物标准品格列吡嗪、格列齐特、格列本脲和格列美脲进行了测定，比较了线性、准确性、精密度和检测限（LOD）。所有实验均在反相柱上进行，以水和乙腈为流动相。对于每种检测方法，均在相同的色谱条件下进行分离。结果表明，与 UV 检测和 ELSD 相比，CAD 产生了几乎均匀的响应。与 3 种检测器相比，它具有最佳的准确性和 LOD，在较高浓度下与 UV 检测具有相似的精密度，而在较低浓度下，CAD 或 ELSD 的精密度优于 UV 检测。事实上，CAD 的 LOD 可以比 ELSD 高两倍。UV 和 ELSD 的线性度在 R（2）>0.99 时令人满意，尽管在 CAD 的情况下需要进行对数-对数转换。所提出的方法还应用于实际的抗糖尿病药物和糖尿病相关膳食补充剂。
