Atatürk University, Faculty of Science, Biology Department, 25240 Erzurum, Turkey.
Food Chem Toxicol. 2012 Jun;50(6):2142-8. doi: 10.1016/j.fct.2011.09.018. Epub 2011 Sep 19.
The present experiment was undertaken to determine the effectiveness of propolis in alleviating the toxicity of TCDD on cultured primary rat hepatocytes. Propolis (25, 50 and 100 μM) was added to plain culture or simultaneously with TCDD (5 and 10 μM). The hepatocytes were treated with TCDD and propolis for 48 h. Then cell viability was detected by [3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide] (MTT) assay and lactate dehydrogenase (LDH) release, while total antioxidant capacity (TAC) and total oxidative stress (TOS) levels were determined to evaluate the oxidative injury. The DNA damage was also analyzed by liver micronucleus assay (LMN) and 8-oxo-2-deoxyguanosine (8-OH-dG). The results of MTT and LDH assays showed that TCDD decreased cell viability. TCDD also increased TOS level and decreased TAC level in rat hepatocytes. On the basis of increasing doses, the TCDD caused significant increases of micronucleated hepatocytes (MNHEPs) and 8-OH-dG levels as compared to control culture. In cultures treated with propolis alone, cell viability and TOS level were not affected, while the level of TAC was significantly increased in dose-dependent fashion. The presence of propolis with TCDD modulated its toxic effects on primary hepatocytes cultures. Noteworthy, propolis has a protective effect against TCDD-mediated DNA damages.
本实验旨在确定蜂胶对 TCDD 诱导的原代大鼠肝细胞毒性的缓解作用。蜂胶（25、50 和 100 μM）分别加入普通培养物或与 TCDD（5 和 10 μM）同时加入。用 TCDD 和蜂胶处理肝细胞 48 小时。然后通过 [3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐]（MTT）测定法和乳酸脱氢酶（LDH）释放检测细胞活力，同时测定总抗氧化能力（TAC）和总氧化应激（TOS）水平以评估氧化损伤。还通过肝微核试验（LMN）和 8-氧代-2-脱氧鸟苷（8-OH-dG）分析 DNA 损伤。MTT 和 LDH 测定结果表明 TCDD 降低了细胞活力。TCDD 还增加了大鼠肝细胞的 TOS 水平并降低了 TAC 水平。基于增加的剂量，与对照培养相比，TCDD 导致微核化肝细胞（MNHEPs）和 8-OH-dG 水平显著增加。单独用蜂胶处理的培养物中，细胞活力和 TOS 水平不受影响，而 TAC 水平则呈剂量依赖性显著增加。蜂胶与 TCDD 共同存在调节了其对原代肝细胞培养物的毒性作用。值得注意的是，蜂胶对 TCDD 介导的 DNA 损伤具有保护作用。
