蜂胶通过调节氧化参数对四氯化碳诱导的肝损伤和肾损伤的抗氧化活性及保护作用。
Antioxidant activity and protective effect of propolis against carbon tetrachloride-induced liver and kidney injury by modulation of oxidative parameters.
作者信息
El-Haskoury Redouan, Al-Waili Noori, Kamoun Zeineb, Makni Mohamed, Al-Waili Ahmed, Lyoussi Badiaa
机构信息
Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health, and Quality of Life (SNAMOPEQ), Department of Biology, Faculty of Sciences Dhar Mehraz, Sidi Mohamed Ben Abdellah University, Fez 30000, Morocco.
New York Medical Care for Nephrology, Richmond Hill, New York, United States.
出版信息
Vet World. 2021 Dec;14(12):3076-3083. doi: 10.14202/vetworld.2021.3076-3083. Epub 2021 Dec 8.
BACKGROUND AND AIM
Propolis has a protective effect against cellular damage caused by toxic agents such as drugs, metals, xenobiotics, and chemicals. The aim of this study was to investigate the antioxidant activity and the effect of ethanolic extract of propolis on carbon tetrachloride (CCl4)-induced oxidative stress on kidney and liver injury in rat.
MATERIALS AND METHODS
The study quantified phenol, flavone, and flavonol in propolis and assessed antioxidant activity using 2, 2-diphenyl-1-picrylhydrazyl, ferric reducing antioxidant power, and molybdate. The investigators used four groups of rats to study the effect of propolis on CCl-induced toxicity. Propolis extract was given orally (500 mg/kg) for 12 days, and CCl1 mL/kg) was administered intraperitoneally on day 5 of the experiment. Blood and tissue samples of the liver and kidney were collected on day 13 to measure biochemical and oxidative parameters. The parameters included malondialdehyde (MDA), protein carbonyl formation (PCO), advanced oxidation protein products (AOPP), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH), and ascorbic acid (AA). Biochemical parameters included liver enzymes, blood urea (BU), creatinine, and uric acid (UA).
RESULTS
CCl decreased antioxidant agents, including CAT, GPx, GSH, and AA in the liver and kidney tissues. The oxidative agents' levels, including MDA, PCO, and AOPP, increased by CCl compared to the control group. CCl increased liver enzymes, UA, BU, and creatinine in the blood samples. Propolis significantly alleviated liver and kidney function, improved antioxidant parameters, and decreased levels of oxidative agents.
CONCLUSION
The data showed for the 1 time that Moroccan propolis has a protective effect against CCl-induced kidney and liver toxicity by maintaining the activity of the antioxidant defense system, which was most likely due to its antioxidant activity.
背景与目的
蜂胶对药物、金属、异生物素和化学物质等有毒物质引起的细胞损伤具有保护作用。本研究旨在探讨蜂胶乙醇提取物的抗氧化活性及其对四氯化碳（CCl₄）诱导的大鼠肾和肝损伤氧化应激的影响。
材料与方法
本研究对蜂胶中的酚类、黄酮类和黄酮醇类进行了定量分析，并使用2,2-二苯基-1-苦基肼、铁还原抗氧化能力和钼酸盐评估了抗氧化活性。研究人员使用四组大鼠来研究蜂胶对CCl₄诱导毒性的影响。口服给予蜂胶提取物（500mg/kg），持续12天，并在实验的第5天腹腔注射CCl₄（1mL/kg）。在第13天采集肝脏和肾脏的血液及组织样本，以测量生化和氧化参数。这些参数包括丙二醛（MDA）、蛋白质羰基形成（PCO）、晚期氧化蛋白产物（AOPP）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、还原型谷胱甘肽（GSH）和抗坏血酸（AA）。生化参数包括肝酶、血尿素（BU）、肌酐和尿酸（UA）。
结果
CCl₄降低了肝脏和肾脏组织中的抗氧化剂，包括CAT、GPx、GSH和AA。与对照组相比，CCl₄使包括MDA、PCO和AOPP在内的氧化剂水平升高。CCl₄使血液样本中的肝酶、UA、BU和肌酐升高。蜂胶显著改善了肝脏和肾脏功能，改善了抗氧化参数，并降低了氧化剂水平。
结论
数据首次表明，摩洛哥蜂胶通过维持抗氧化防御系统的活性，对CCl₄诱导的肾和肝毒性具有保护作用，这很可能归因于其抗氧化活性。
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