Department of Dermatology, Keimyung University School of Medicine, Daegu, Republic of Korea.
Int J Mol Med. 2012 Jan;29(1):32-6. doi: 10.3892/ijmm.2011.803. Epub 2011 Sep 30.
Platelet-rich plasma (PRP) is derived from fresh whole blood, which contains a high concentration of platelets. Recently, PRP has been used for skin wound healing and rejuvenation. However, the molecular mechanisms underlying PRP-inducing wound healing processes are still largely unknown. The aim of this study is to evaluate the effect of PRP on the expression of G1 cell cycle regulatory proteins, type I collagen, matrix metalloproteinase-1 (MMP-1), and MMP-2 in human skin fibroblasts (HSF). We performed a cell proliferation and a migration assay, immunoblotting, and a chloramphenicol acetyltransferase (CAT) assay in PRP-treated human skin fibroblasts. PRP treatment induced increased rates of cell proliferation and cell migration. Expression of cyclin A protein was increased by a low concentration (0.5%) of PRP-treated HSF. In addition, expression of Rb, cyclin E, and cyclin-dependent kinase 4 proteins was increased by a high concentration (5%) of PRP-treated HSF. High concentration of PRP induced an up-regulation of type I collagen, MMP-1, and MMP-2 expression in HSF. Taken together, PRP treatment induced an increase in expression of G1 cell cycle regulators, type I collagen and MMP-1, thereby accelerating the wound healing process.
富含血小板的血浆（PRP）源自新鲜全血，其中含有高浓度的血小板。最近，PRP 已被用于皮肤创伤愈合和修复。然而，PRP 诱导创伤愈合过程的分子机制在很大程度上仍不清楚。本研究旨在评估 PRP 对人皮肤成纤维细胞（HSF）中 G1 细胞周期调控蛋白、I 型胶原、基质金属蛋白酶-1（MMP-1）和 MMP-2 表达的影响。我们在 PRP 处理的人皮肤成纤维细胞中进行了细胞增殖和迁移测定、免疫印迹和氯霉素乙酰转移酶（CAT）测定。PRP 处理诱导细胞增殖和迁移率增加。低浓度（0.5%）PRP 处理的 HSF 中环素 A 蛋白的表达增加。此外，高浓度（5%）PRP 处理的 HSF 中 Rb、细胞周期蛋白 E 和细胞周期蛋白依赖性激酶 4 蛋白的表达增加。高浓度的 PRP 诱导 HSF 中 I 型胶原、MMP-1 和 MMP-2 表达的上调。总之，PRP 处理诱导 G1 细胞周期调节剂、I 型胶原和 MMP-1 的表达增加，从而加速创伤愈合过程。
