Department of Genetic Engineering, Kinki University, Kinokawa, Wakayama, Japan.
Biochem Genet. 2012 Apr;50(3-4):269-76. doi: 10.1007/s10528-011-9469-x. Epub 2011 Oct 1.
Carbonic anhydrases are conserved in vertebrates and invertebrates, and a noncatalytic carbonic anhydrase-related protein VIII (CARP VIII) has been found in deuterostomes and the phylum Placozoa. I isolated a cDNA encoding a noncatalytic CARP from the mantle of the pearl oyster Pinctada fucata. The polypeptide (CARP-1) predicted from the nucleotide sequence shares 44-60% identity with known CARP VIII sequences, and its phylogenetic analysis showed that P. fucata formed a single group with deuterostome invertebrates. However, since CARP VIII sequences are not identified in protostomes, these results suggest that CARP-1 may have originated in molluscs independently from deuterostome CARP VIII sequences.
碳酸酐酶在脊椎动物和无脊椎动物中是保守的，并且在后生动物和多孔动物门中发现了一种非催化碳酸酐酶相关蛋白 VIII（CARP VIII）。我从珍珠贝 Pinctada fucata 的套膜中分离出编码非催化 CARP 的 cDNA。从核苷酸序列预测的多肽（CARP-1）与已知的 CARP VIII 序列具有 44-60%的同一性，其系统发育分析表明，P. fucata 与后生动物无脊椎动物形成了一个单独的组。然而，由于在原口动物中未鉴定出 CARP VIII 序列，这些结果表明 CARP-1 可能独立于后生动物 CARP VIII 序列起源于软体动物。
