Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China.
Fish Shellfish Immunol. 2012 Sep;33(3):652-8. doi: 10.1016/j.fsi.2012.06.026. Epub 2012 Jul 13.
TRAF3 is a highly versatile regulator that negatively regulates JNK and alternative nuclear factor-κB signalling, but positively controls type I interferon production. To investigate TRAF3 function in innate immune responses among invertebrate especially mollusk, we characterized TRAF3 (PfTRAF3) from pearl oyster Pinctada fucata, one of the most important bivalve mollusks for seawater pearl production. PfTRAF3 cDNA is 2261 bp with an open reading frame of 1623 bp encoding a putative protein of 541 amino acids. The deduced PfTRAF3 contains a RING finger domain, two TRAF domains with zinc finger domains and a conserved C-terminal meprin and TRAF homology (MATH) domain. Comparison and phylogenetic analysis revealed that PfTRAF3 from mollusk shared a higher identity with Ciona intestinalis TRAF3 from urochordata, Branchiostoma belcheri TRAF3 from cephalochordate, and even TRAF3 from vertebrate than with insect homologues. Furthermore, gene expression analyses suggested that PfTRAF3 was involved in the immune response to Vibrio alginolyticus.
TRAF3 是一种高度通用的调节剂，可负向调节 JNK 和替代性核因子-κB 信号，但正向控制 I 型干扰素的产生。为了研究 TRAF3 在无脊椎动物，特别是软体动物中先天免疫反应中的功能，我们从珍珠贝（Pinctada fucata）中鉴定了 TRAF3（PfTRAF3），珍珠贝是最重要的海水珍珠生产双壳贝类之一。PfTRAF3 cDNA 长 2261bp，开放阅读框为 1623bp，编码一个推测的 541 个氨基酸的蛋白质。推导的 PfTRAF3 包含一个 RING 指结构域、两个具有锌指结构域的 TRAF 结构域和一个保守的 C 末端髓过氧化物酶和 TRAF 同源（MATH）结构域。比较和系统发育分析表明，软体动物 PfTRAF3 与尾索动物文昌鱼（Ciona intestinalis）TRAF3、头索动物文昌鱼（Branchiostoma belcheri）TRAF3 的同源性更高，甚至与脊椎动物的 TRAF3 比与昆虫同源物的同源性更高。此外，基因表达分析表明，PfTRAF3 参与了对溶藻弧菌的免疫反应。
