Wang S S, Zakian V A
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Mol Cell Biol. 1990 Aug;10(8):4415-9. doi: 10.1128/mcb.10.8.4415-4419.1990.
By using T4 DNA polymerase rather than S1 or Bal31 nuclease to clone yeast telomeres, very little telomeric DNA is lost. These clones were used to determine the DNA sequence of virtually the entire telomeric tract. Our results demonstrated that a slightly modified version, C2-3A(CA)1-6, of the consensus derived from sequence analysis of more-internal regions (J. Shampay, J. W. Szostak, and E. H. Blackburn, Nature [London] 310:154-157, 1984) extends to the very end of the chromosome. The sequence analysis also suggests that yeast telomeres consist of two domains: the proximal 120 to 150 base pairs, which appear to be protected from processes such as recombination, degradation, and elongation, and the distal portion of the telomere, which is more susceptible to these events.
通过使用T4 DNA聚合酶而非S1或Bal31核酸酶来克隆酵母端粒，端粒DNA几乎没有丢失。这些克隆用于确定几乎整个端粒区域的DNA序列。我们的结果表明，从更多内部区域的序列分析得出的共有序列（J. 尚佩、J. W. 绍斯塔克和E. H. 布莱克本，《自然》[伦敦] 310:154 - 157，1984）的一个稍微修改的版本C2 - 3A(CA)1 - 6延伸到了染色体的最末端。序列分析还表明酵母端粒由两个结构域组成：近端的120至150个碱基对，似乎受到保护而免受诸如重组、降解和延伸等过程的影响；以及端粒的远端部分，它更容易受到这些事件的影响。
