Van der Ploeg L H, Liu A Y, Borst P
Cell. 1984 Feb;36(2):459-68. doi: 10.1016/0092-8674(84)90239-3.
We have developed a method for the molecular cloning of DNA adjacent to chromosome ends (telomeres). A recombinant DNA clone obtained from the telomeres of the protozoan Trypanosoma brucei contains large stretches of the repeat (CCCTAA)n. This repeat is flanked by a larger subtelomeric repeat (29 bp in one case). These repeats account for the presence of large DNA stretches not cut by restriction enzymes downstream of telomeric VSG genes. All telomeres analyzed thus far (more than 30) grow by approximately 6 bp per trypanosomal division and contract by occasional large deletions. Our results suggest that growth is due mainly to addition of CCCTAA units.
我们已经开发出一种用于分子克隆与染色体末端（端粒）相邻的DNA的方法。从原生动物布氏锥虫的端粒获得的一个重组DNA克隆含有大片段的（CCCTAA）n重复序列。这个重复序列两侧是一个更大的亚端粒重复序列（在一个例子中为29bp）。这些重复序列解释了端粒VSG基因下游未被限制性酶切割的大片段DNA的存在。迄今为止分析的所有端粒（超过30个）在每个锥虫分裂周期中大约增长6bp，并偶尔因大的缺失而收缩。我们的结果表明，增长主要是由于CCCTAA单位的添加。
