Department of Community Health Sciences, College of Applied Medical Sciences, King Saud University, PO Box 10219, Riyadh 11433, Saudi Arabia.
J Nat Med. 2012 Apr;66(2):302-10. doi: 10.1007/s11418-011-0590-2. Epub 2011 Oct 1.
The present study was designed to investigate the antihypertensive effect of Melothria maderaspatana leaf fractions on deoxycorticosterone acetate (DOCA)-salt-induced hypertensive rats and to identify compounds from the active fraction by GC-MS analysis. Administration of DOCA salt significantly increased the systolic and diastolic blood pressure compared to sham-operated control rats. When treated with chloroform (CFM), ethyl acetate (EAFM) or methanol fractions of M. maderaspatana (MFM), EAFM alone significantly lowered the systolic and diastolic blood pressure. The levels of magnesium and copper significantly increased in plasma and decreased in tissues while the zinc level significantly increased in plasma and tissues, and administration of EAFM brought these parameters back to sham-operated control levels. By GC-MS analysis, phytochemicals such as coumarin, vallinic acid, p-coumaric acid, gallic acid, caffeic acid, and ferulic acid were identified in EAFM. In conclusion, the EAFM controls blood pressure in DOCA-salt hypertensive rats and reverts the metabolic alterations in magnesium, copper and zinc.
本研究旨在探讨麻疯树叶部分对去氧皮质酮醋酸盐（DOCA）-盐诱导的高血压大鼠的降压作用，并通过 GC-MS 分析鉴定活性部分的化合物。与假手术对照大鼠相比，给予 DOCA 盐可显著升高收缩压和舒张压。用氯仿（CFM）、乙酸乙酯（EAFM）或甲醇部分处理麻疯树（MFM）时，单独的 EAFM 可显著降低收缩压和舒张压。血浆中镁和铜的水平显著升高，组织中镁和铜的水平降低，而锌的水平在血浆和组织中显著升高，给予 EAFM 可使这些参数恢复到假手术对照水平。通过 GC-MS 分析，在 EAFM 中鉴定出香豆素、缬草酸、对香豆酸、没食子酸、咖啡酸和阿魏酸等植物化学物质。总之，EAFM 可控制 DOCA-盐性高血压大鼠的血压，并逆转镁、铜和锌的代谢改变。
