Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, NH 03755, USA.
J Bacteriol. 2011 Dec;193(23):6770-80. doi: 10.1128/JB.05929-11. Epub 2011 Sep 30.
Pseudomonas aeruginosa is an opportunistic pathogen capable of group behaviors, including biofilm formation and swarming motility. These group behaviors are regulated by both the intracellular signaling molecule c-di-GMP and acylhomoserine lactone quorum-sensing systems. Here, we show that the Pseudomonas quinolone signal (PQS) system also contributes to the regulation of swarming motility. Specifically, our data indicate that 2-heptyl-4-quinolone (HHQ), a precursor of PQS, likely induces the production of the phenazine-1-carboxylic acid (PCA), which in turn acts via an as-yet-unknown downstream mechanism to repress swarming motility. We show that this HHQ- and PCA-dependent swarming repression is apparently independent of changes in global levels of c-di-GMP, suggesting complex regulation of this group behavior.
铜绿假单胞菌是一种机会性病原体，能够进行群体行为，包括生物膜形成和群集运动。这些群体行为受到胞内信号分子 c-di-GMP 和酰基高丝氨酸内酯群体感应系统的调节。在这里，我们表明，铜绿假单胞菌喹诺酮信号（PQS）系统也有助于调节群集运动。具体而言，我们的数据表明，2-庚基-4-喹诺酮（HHQ），PQS 的前体，可能诱导吩嗪-1-羧酸（PCA）的产生，PCA 反过来通过一个尚未知的下游机制来抑制群集运动。我们表明，这种依赖于 HHQ 和 PCA 的群集抑制显然与 c-di-GMP 的全局水平变化无关，表明对这种群体行为的复杂调节。
