School of Biological Sciences, Flinders University, Adelaide, South Australia, Australia.
PLoS One. 2011;6(9):e25173. doi: 10.1371/journal.pone.0025173. Epub 2011 Sep 23.
Environmental parameters drive phenotypic and genotypic frequency variations in microbial communities and thus control the extent and structure of microbial diversity. We tested the extent to which microbial community composition changes are controlled by shifting physiochemical properties within a hypersaline lagoon. We sequenced four sediment metagenomes from the Coorong, South Australia from samples which varied in salinity by 99 Practical Salinity Units (PSU), an order of magnitude in ammonia concentration and two orders of magnitude in microbial abundance. Despite the marked divergence in environmental parameters observed between samples, hierarchical clustering of taxonomic and metabolic profiles of these metagenomes showed striking similarity between the samples (>89%). Comparison of these profiles to those derived from a wide variety of publically available datasets demonstrated that the Coorong sediment metagenomes were similar to other sediment, soil, biofilm and microbial mat samples regardless of salinity (>85% similarity). Overall, clustering of solid substrate and water metagenomes into discrete similarity groups based on functional potential indicated that the dichotomy between water and solid matrices is a fundamental determinant of community microbial metabolism that is not masked by salinity, nutrient concentration or microbial abundance.
环境参数驱动微生物群落的表型和基因型频率变化，从而控制微生物多样性的程度和结构。我们测试了微生物群落组成变化在多大程度上受到盐度变化 99 实用盐度单位 (PSU)、氨浓度变化一个数量级和微生物丰度变化两个数量级的控制。尽管在样本之间观察到环境参数的显著差异，但对这些宏基因组的分类和代谢谱进行层次聚类表明，样本之间具有惊人的相似性 (>89%)。将这些图谱与来自各种公共可用数据集的图谱进行比较表明，无论盐度如何 (>85%的相似性)，科朗沉积物宏基因组与其他沉积物、土壤、生物膜和微生物垫样本相似。总体而言，基于功能潜力将固体基质和水宏基因组聚类为离散相似组表明，水和固体基质之间的二分法是社区微生物代谢的基本决定因素，不受盐度、营养浓度或微生物丰度的影响。
