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基于 16S rRNA 测序的微生物组成分析:一种用于确定考古赭石来源的概念验证方法。

Microbial composition analyses by 16S rRNA sequencing: A proof of concept approach to provenance determination of archaeological ochre.

机构信息

College of Science and Engineering, Flinders University, Adelaide, South Australia, Australia.

Chemistry and Physics, Arcadia University, Glenside, PA United States of America.

出版信息

PLoS One. 2017 Oct 18;12(10):e0185252. doi: 10.1371/journal.pone.0185252. eCollection 2017.

DOI:10.1371/journal.pone.0185252
PMID:29045459
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5646784/
Abstract

Many archaeological science studies use the concept of "provenance", where the origins of cultural material can be determined through physical or chemical properties that relate back to the origins of the material. Recent studies using DNA profiling of bacteria have been used for the forensic determination of soils, towards determination of geographic origin. This manuscript presents a novel approach to the provenance of archaeological minerals and related materials through the use of 16S rRNA sequencing analysis of microbial DNA. Through the microbial DNA characterization from ochre and multivariate statistics, we have demonstrated the clear discrimination between four distinct Australian cultural ochre sites.

摘要

许多考古科学研究都使用“产地”这一概念,通过与物质起源相关的物理或化学特性来确定文化材料的起源。最近,人们还利用细菌 DNA 分析技术来进行法医鉴定土壤,以确定其地理起源。本文提出了一种通过对微生物 DNA 进行 16S rRNA 测序分析来确定考古矿物及相关材料产地的新方法。通过对赤铁矿和多元统计数据中的微生物 DNA 进行特征分析,我们已经证明了澳大利亚四个不同的文化赤铁矿产地之间的明显区别。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1315/5646784/b62277adb3b1/pone.0185252.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1315/5646784/3e55bd3c7302/pone.0185252.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1315/5646784/fcdbdc0d7fc4/pone.0185252.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1315/5646784/4437452a9f29/pone.0185252.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1315/5646784/b62277adb3b1/pone.0185252.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1315/5646784/3e55bd3c7302/pone.0185252.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1315/5646784/fcdbdc0d7fc4/pone.0185252.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1315/5646784/4437452a9f29/pone.0185252.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1315/5646784/b62277adb3b1/pone.0185252.g004.jpg

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