Wallenberg Neuroscience Center, Lund University, Lund, Sweden.
Epilepsia. 2011 Oct;52 Suppl 8:16-8. doi: 10.1111/j.1528-1167.2011.03226.x.
Optogenetics allow for timely precise and cell-population specific activation or inhibition of neuronal activity. Hallorhodopsin NpHR is an inward cholride pump, which when activated by orange light, hyperpolarises neuronal membrane and inhibitis action potential generation. We were first to show that transduction of principal neurons with NpHR in organotypic hippocampal slice cultures effectivey attenuates epileptiform activity when exposed to orange light. Our data suggest that optogenetic approach may become a useful method for controlling epileptiform activity, and may open novel avenues to develop alternative treatment strategies for epilepsy.
光遗传学允许及时、精确和针对细胞群体的神经元活动的激活或抑制。Hallorhodopsin NpHR 是一种内向氯离子泵，当被橙色光激活时，使神经元膜超极化并抑制动作电位的产生。我们首先证明，在器官型海马切片培养物中，将 NpHR 转导到主神经元中，当暴露于橙色光时，可有效抑制癫痫样活动。我们的数据表明，光遗传学方法可能成为控制癫痫样活动的一种有用方法，并可能为开发治疗癫痫的替代治疗策略开辟新途径。
