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通过核苷酸测序和限制性图谱分析噬菌体BF23 DNA末端重复序列中的早期前基因

Characterization of preearly genes in the terminal repetition of bacteriophage BF23 DNA by nucleotide sequencing and restriction mapping.

作者信息

Wiest J S, McCorquodale D J

机构信息

Department of Biochemistry, Medical College of Ohio, Toledo 43699.

出版信息

Virology. 1990 Aug;177(2):745-54. doi: 10.1016/0042-6822(90)90541-x.

Abstract

A finer restriction map of the terminal repetition of bacteriophage BF23 DNA was determined and used to localize a 3.4-kbp deletion in the terminal repetition and to determine the physical location of preearly gene A2-A3. The nucleotide sequence of gene A2-A3 was determined and shown to code for a protein of 125 amino acids with no indication of a membrane transport sequence. The beginning of an adjacent gene, probably gene A1, was also sequenced.

摘要

确定了噬菌体BF23 DNA末端重复序列更精细的限制酶切图谱,并用于定位末端重复序列中的一个3.4千碱基对的缺失,以及确定早期前基因A2 - A3的物理位置。测定了基因A2 - A3的核苷酸序列,结果表明它编码一个含125个氨基酸的蛋白质,未显示有膜转运序列的迹象。还对相邻基因(可能是基因A1)的起始部分进行了测序。

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