Okada K
Gene. 1980 Mar;8(4):369-90. doi: 10.1016/0378-1119(80)90042-6.
Using 13 deletion mutants of bacteriophage BF23, physical as well as genetic structures of that portion of the genome which is dispensable for phage growth were investigated. The dispensable region covers at least 15% of the genome of wild type BF23, extending from about 0.2 to 0.35 map unit. Restriction endonuclease (EcoRI and HindIII) cleavage sites and the sites of single-strand interruptions in this dispensable region were localized. It was found that the dispensable region contains an interruption site, which is missing in the mutant BF23st(0) used by Okada and Shimura (1980). Wild-type phage DNA is heterogeneous in the presence or absence of specific single-strand interruptions in this or in a neighboring region of the genome.
利用噬菌体BF23的13个缺失突变体，对噬菌体生长非必需的那部分基因组的物理结构和遗传结构进行了研究。非必需区域至少覆盖野生型BF23基因组的15%，从约0.2到0.35个图距单位。在这个非必需区域定位了限制性内切酶（EcoRI和HindIII）的切割位点以及单链中断位点。发现非必需区域包含一个中断位点，冈田和志村（1980年）使用的突变体BF23st(0)中没有该位点。野生型噬菌体DNA在基因组的这个区域或相邻区域存在或不存在特定单链中断的情况下是异质的。
