Gyorkos T W, Genta R M, Viens P, MacLean J D
Div. of Clinical Epidemiology, Montreal General Hospital, Quebec, Canada.
Am J Epidemiol. 1990 Aug;132(2):257-64. doi: 10.1093/oxfordjournals.aje.a115655.
As part of a screening and treatment program for intestinal parasite infections offered to newly arrived Southeast Asian refugees in Canada between July 1982 and February 1983, a total of 232 sera were tested for Strongyloides infection using an enzyme-linked immunosorbent assay (immunoglobulin G). These results were compared with coprologic results and eosinophil counts. The seroprevalence was 76.6% (131 of 171) among Kampucheans, 55.6% (15 of 27) among Laotians, and 11.8% (4 of 34) among Vietnamese. A statistically significant relation (p less than 0.001) was found between Strongyloides serology and Strongyloides infection on stool examination (prevalence, 24.7%) among Kampucheans. Eosinophilia (greater than or equal to 10%) was found to be significantly associated with both infection measures. Using coprologic results as the "gold standard," the properties of the serologic test were estimated to be: sensitivity (95%), specificity (29%), positive predictive value (30%), and negative predictive value (95%). These estimates should be regarded as minimal values, as stool examination for Strongyloides infection can be an unreliable diagnostic reference. Further evaluation of the discrepancies observed between coprologic and serologic testing is required to determine the usefulness of these tests in epidemiologic studies.
作为1982年7月至1983年2月期间为加拿大新抵达的东南亚难民提供的肠道寄生虫感染筛查和治疗项目的一部分，总共232份血清采用酶联免疫吸附测定法（免疫球蛋白G）检测类圆线虫感染。将这些结果与粪便检查结果和嗜酸性粒细胞计数进行比较。柬埔寨人的血清阳性率为76.6%（171人中的131人），老挝人为55.6%（27人中的15人），越南人为11.8%（34人中的4人）。在柬埔寨人中，类圆线虫血清学与粪便检查中类圆线虫感染（患病率为24.7%）之间存在统计学显著关系（p小于0.001）。嗜酸性粒细胞增多（大于或等于10%）与两种感染指标均显著相关。以粪便检查结果作为“金标准”，血清学检测的特性估计为：敏感性（95%）、特异性（29%）、阳性预测值（30%）和阴性预测值（95%）。这些估计值应被视为最小值，因为类圆线虫感染的粪便检查可能是不可靠的诊断参考。需要进一步评估粪便检查和血清学检测之间观察到的差异，以确定这些检测在流行病学研究中的有用性。
