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开发一种使用指尖少量采集的血液检测七种生化指标、HbA1c 和红细胞压积的方法。

Development of an assay of seven biochemical items, HbA1c, and hematocrit using a small amount of blood collected from the fingertip.

机构信息

Physical Screening, Inc., Tokyo, Japan.

出版信息

Clin Chim Acta. 2012 Jan 18;413(1-2):192-7. doi: 10.1016/j.cca.2011.09.021. Epub 2011 Sep 22.

DOI:10.1016/j.cca.2011.09.021
PMID:21968067
Abstract

BACKGROUND

Lifestyle-related diseases in Japan account for 30% of the entire medical expenditure of the country and cause 60% of all deaths. For the prevention of lifestyle-related diseases, medical examination by laboratory tests on metabolic syndrome is important.

METHODS

To undertake examination by collection of blood from a fingertip, we developed the "Well Kit". About 65 μl of blood collected from a fingertip was diluted with buffer solution, which contained two internal standard materials. The kit also separated corpuscles and diluted plasma with a special filter. It measured the obtained diluted plasma using the JCA-BM2250.

RESULTS

This measurement system was evaluated for the quantitative analysis of 8 items. The uncertainties of tested items of this measurement system were 1.7% to 6.4%. The coefficients of correlation of all tested items between this measurement value and the venous plasma sample value were 0.876-0.991, and hematocrit was 0.958.

CONCLUSIONS

This system for testing blood collected from a fingertip is simple to use and can be applied in testing for metabolic syndrome. In addition, this testing system is useful in the medical examination of the personal healthcare and inhabitants.

摘要

背景

在日本,与生活方式有关的疾病占国家全部医疗支出的 30%,导致 60%的死亡。为了预防与生活方式有关的疾病,通过实验室检测代谢综合征进行体检非常重要。

方法

为了从指尖采集血液进行检查,我们开发了“健康试剂盒”。从指尖采集约 65μl 的血液,用含有两种内标物质的缓冲液稀释。试剂盒还使用特殊的过滤器分离血细胞并稀释血浆。使用 JCA-BM2250 测量获得的稀释血浆。

结果

该测量系统用于对 8 项进行定量分析。该测量系统的测试项目的不确定度为 1.7%至 6.4%。该测量值与静脉血浆样本值之间所有测试项目的相关系数为 0.876-0.991,血细胞比容为 0.958。

结论

这种从指尖采集血液的检测系统使用简便,可用于检测代谢综合征。此外,该检测系统在个人健康护理和居民的体检中非常有用。

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