Huijskens M J A J, Castel R, Vermeer H J, Verheijen F M
Department of Clinical Chemistry, Albert Schweitzer Hospital, Dordrecht, the Netherlands.
Department of Clinical Chemistry, Result Laboratorium C.V., Dordrecht, the Netherlands.
Pract Lab Med. 2019 Sep 19;17:e00135. doi: 10.1016/j.plabm.2019.e00135. eCollection 2019 Nov.
The aim of this study was to determine whether the Hem-Col method of obtaining and storing blood is an acceptable alternative to venepuncture for measuring Diabetes Care parameters. : Hem-Col is a novel blood collection device that is designed to collect capillary blood drawn with a finger prick. Hem-Col is a microtube containing an anticoagulant and a preservation buffer to enhance analyte stability in whole blood. The Diabetes Care parameters cholesterol, creatinine, HbA1c, high density lipoprotein (HDL) cholesterol, low density lipoprotein (LDL) cholesterol, and triglycerides were measured both in blood/plasma collected via Hem-Col and blood/plasma collected with venepuncture. The results were compared to assess the agreement between the two methods. : HbA1c shows agreement after storage for up to 120 hours at temperatures ranging from 4 to 37 °C. Cholesterol, HDL cholesterol, LDL cholesterol, triglycerides and creatinine can be measured after 120 hours of storage in Hem-Col buffer, if high temperatures are avoided, and with the use of correction factors or adaptations to reported reference intervals. : Hem-Col is suitable for the measurement of HbA1c after storage for up to 120 hours at temperatures ranging from 4 to 37 °C. Cholesterol, creatinine, HDL cholesterol, LDL cholesterol and triglycerides can be measured after 120 hours of storage in Hem-Col buffer, if high temperatures are avoided. Further studies are required to determine whether Hem-Col can replace the venepuncture for the Diabetes Care parameters.
本研究的目的是确定采用Hem-Col方法采集和储存血液是否是一种可接受的替代静脉穿刺来测量糖尿病护理参数的方法。：Hem-Col是一种新型采血装置，设计用于采集手指针刺抽取的毛细血管血。Hem-Col是一种微管，含有抗凝剂和保存缓冲液，以提高全血中分析物的稳定性。对通过Hem-Col采集的血液/血浆和通过静脉穿刺采集的血液/血浆中的糖尿病护理参数胆固醇、肌酐、糖化血红蛋白（HbA1c）、高密度脂蛋白（HDL）胆固醇、低密度脂蛋白（LDL）胆固醇和甘油三酯进行了测量。比较结果以评估两种方法之间的一致性。：在4至37°C的温度下储存长达120小时后，HbA1c显示出一致性。如果避免高温，并使用校正因子或调整报告的参考区间，胆固醇、HDL胆固醇、LDL胆固醇、甘油三酯和肌酐可在Hem-Col缓冲液中储存120小时后进行测量。：Hem-Col适用于在4至37°C的温度下储存长达120小时后测量HbA1c。如果避免高温，胆固醇、肌酐、HDL胆固醇、LDL胆固醇和甘油三酯可在Hem-Col缓冲液中储存120小时后进行测量。需要进一步研究以确定Hem-Col是否可以替代静脉穿刺来测量糖尿病护理参数。
