Junior Research Group Antibody Technologies, Department of Biotechnology, Institute of Biochemistry and Biology, Potsdam University, Karl-Liebknecht-Strasse 24-25, D-14476 Potsdam-Golm, Germany.
J Biotechnol. 2011 Dec 10;156(3):173-81. doi: 10.1016/j.jbiotec.2011.09.002. Epub 2011 Sep 29.
The production of monoclonal antibodies by hybridoma technology is dependent on lymphocytes taken from vertebrates which have to be immunized against the corresponding antigen. We present here our first experiments which should allow the replacement of this in vivo immunization step by an in vitro immunization procedure. This work provides new possibilities for the specific activation of immune cells in order to use them for the generation of antibodies which are not of murine origin. Bone marrow-derived dendritic cells were loaded with antigen and co-cultured with naïve T and B lymphocytes of non-immunized mice. The interaction and activation of the different cell types were investigated by measuring the expression of specific cell surface markers, the release of activation-dependent interleukins and the secretion of antigen-specific antibodies. We could demonstrate that dendritic cells process and present antigen fragments and activate T cells, that T cells proliferate and release activation-induced interleukins, and that B cells maturate under the influence of activated T cells and secrete antigen-specific antibodies.
杂交瘤技术生产单克隆抗体依赖于针对相应抗原进行免疫的脊椎动物淋巴细胞。我们在此介绍我们的首次实验，这些实验应该可以替代体内免疫步骤，采用体外免疫程序。这项工作为特异性激活免疫细胞提供了新的可能性，以便利用这些细胞生成非鼠源性的抗体。骨髓来源的树突状细胞负载抗原，并与未免疫小鼠的幼稚 T 和 B 淋巴细胞共培养。通过测量特定细胞表面标志物的表达、释放激活依赖性白细胞介素以及分泌抗原特异性抗体来研究不同细胞类型的相互作用和激活。我们证明了树突状细胞处理和呈递抗原片段并激活 T 细胞，T 细胞增殖并释放激活诱导的白细胞介素，并且在激活的 T 细胞的影响下 B 细胞成熟并分泌抗原特异性抗体。
