Kessler W, Heilmaier H, Kreuzer P, Shen J H, Filser M, Filser J G
Gesellschaft für Strahlen- und Umweltforschung, Institut für Toxikologie, Neuherberg, Federal Republic of Germany.
Arch Toxicol. 1990;64(3):242-6. doi: 10.1007/BF02010731.
Male Wistar rats exposed to atmospheric n-hexane excreted in their urine substances which gave rise to absorption spectra like those of pyrroles after the reaction with Ehrlich's reagent. A simple spectrophotometric assay was developed to determine these "pyrrole-like substances" in urine. Their excretion kinetics were evaluated by exposing rats for 8 h to atmospheric n-hexane concentrations between 50 and 3000 ppm. The dose-response curve revealed saturation kinetics according to Michaelis-Menten, Vmax being 1.12 [delta E526.ml urine/8 h n-hexane exposure] and "Km", the atmospheric n-hexane concentration at Vmax/2, being 250 ppm. The excretion of pyrrole-like substances closely correlated with that of 2,5-hexanedione measured by Fedtke and Bolt (1987). Pyrrole-like substances were also found in the urine of a male volunteer. When exposing the person for 3 h to atmospheric n-hexane at a concentration of 146 ppm (equivalent to 55 ppm/8 h) the excreted amount was twice the background value. Due to the sensitivity of this assay it is possible to determine pyrrole-like substances in urine according to the present German MAK or US TLV conditions for n-hexane (50 ppm/8 h).
暴露于大气中正己烷的雄性Wistar大鼠尿液中排泄出一些物质，这些物质与埃利希试剂反应后产生的吸收光谱类似于吡咯的吸收光谱。开发了一种简单的分光光度法来测定尿液中的这些“类吡咯物质”。通过将大鼠暴露于50至3000 ppm的大气中正己烷浓度8小时来评估它们的排泄动力学。剂量反应曲线显示符合米氏动力学的饱和动力学，Vmax为1.12 [ΔE526·ml尿液/8小时正己烷暴露]，“Km”（Vmax/2时的大气中正己烷浓度）为250 ppm。类吡咯物质的排泄与Fedtke和Bolt（1987年）测定的2,5 - 己二酮的排泄密切相关。在一名男性志愿者的尿液中也发现了类吡咯物质。当该人暴露于浓度为146 ppm（相当于55 ppm/8小时）的大气中正己烷3小时时，排泄量是背景值的两倍。由于该检测方法的灵敏度，可以根据目前德国关于正己烷的MAK或美国的TLV条件（50 ppm/8小时）测定尿液中的类吡咯物质。
