Szalay Balázs, Mészáros Gergo, Cseh Áron, Ács Lilla, Deák Magdolna, Kovács László, Vásárhelyi Barna, Balog Attila
First Department of Pediatrics, Semmelweis University, Bókay János Utca 53-54, 1083 Budapest, Hungary.
Clin Dev Immunol. 2012;2012:808724. doi: 10.1155/2012/808724. Epub 2011 Sep 28.
Our aim was to assess the phenotype of T-cell subsets in patients with ankylosing spondylitis (AS), a chronic inflammatory rheumatic disease. In addition, we also tested short-term T-cell activation characteristics. Measurements were done in 13 AS patients before and during the intravenous therapy with anti-TNF agent infliximab (IFX). Flow cytometry was used to determine T-cell subsets in peripheral blood and their intracellular signaling during activation. The prevalence of Th2 and Th17 cells responsible for the regulation of adaptive immunity was higher in AS than in 9 healthy controls. Although IFX therapy improved patients' condition, immune phenotype did not normalize. Cytoplasmic and mitochondrial calcium responses of CD4+ and CD8+ cells to a specific activation were delayed, while NO generation was increased in AS. NO generation normalized sooner upon IFX than calcium response. These results suggest an abnormal immune phenotype with functional disturbances of CD4+ and CD8+ cells in AS.
我们的目的是评估强直性脊柱炎（AS）患者的T细胞亚群表型，AS是一种慢性炎症性风湿性疾病。此外，我们还检测了短期T细胞激活特征。对13例AS患者在静脉注射抗TNF药物英夫利昔单抗（IFX）治疗前和治疗期间进行了检测。采用流式细胞术测定外周血中的T细胞亚群及其激活过程中的细胞内信号传导。与9名健康对照者相比，AS患者中负责调节适应性免疫的Th2和Th17细胞的比例更高。虽然IFX治疗改善了患者的病情，但免疫表型并未恢复正常。AS患者CD4+和CD8+细胞对特异性激活的细胞质和线粒体钙反应延迟，而NO生成增加。IFX治疗后，NO生成比钙反应更快恢复正常。这些结果表明AS患者存在异常免疫表型，伴有CD4+和CD8+细胞的功能障碍。
