IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Folia Microbiol (Praha). 2013 May;58(3):187-93. doi: 10.1007/s12223-012-0196-4. Epub 2012 Sep 25.
Twelve white-rot fungal strains belonging to seven different species were screened on plates under alkaline condition to study the decolourisation of the textile dyes Reactive Black 5 and Poly R-478. Three strains of Trametes versicolor (Micoteca da Universidade do Minho (MUM) 94.04, 04.100 and 04.101) and one strain of Phanerochaete chrysosporium (MUM 94.15) showed better decolourisation results. These four strains were used for decolourisation studies in liquid culture medium. All four selected strains presented more efficient decolourisation rates on Reactive Black 5 than on Poly R-478. For both dyes on solid and liquid culture media, the decolourisation capability exhibited by these strains depended on dye concentration and pH values of the media. Finally, the decolourisation of Reactive Black 5 by T. versicolor strains MUM 94.04 and 04.100 reached 100 %. In addition, the highest white-rot fungi ligninolytic enzyme activities were found for these two strains.
12 株白腐真菌菌株属于 7 个不同的种，在碱性条件下的平板上进行筛选，以研究纺织染料活性黑 5 和 Poly R-478 的脱色。3 株糙皮侧耳（米尼奥大学真菌标本室（MUM）94.04、04.100 和 04.101）和 1 株黄孢原毛平革菌（MUM 94.15）表现出更好的脱色效果。这 4 株菌用于液体培养基中的脱色研究。在液体培养基中，所有 4 株选定的菌株对活性黑 5 的脱色率均高于 Poly R-478。对于固体和液体培养基中的两种染料，这些菌株的脱色能力取决于染料浓度和培养基的 pH 值。最后，T. versicolor 菌株 MUM 94.04 和 04.100 对活性黑 5 的脱色率达到 100%。此外，这两个菌株的白腐真菌木质素酶活性最高。
