Centre for Environmental Engineering Research, Department of Civil Engineering, University of Hong Kong, Hong Kong, China.
Environ Technol. 2011 Aug-Sep;32(11-12):1279-85. doi: 10.1080/09593330.2010.535176.
Effects of Mo(6+) concentration on phototrophic hydrogen production of Rhodobacter sphaeroides were investigated using lactate as the sole carbon source. Results showed that an increase of Mo(6+) from nil to 1000 microg l(-1) led to increases in hydrogen yield, maximum production rate, conversion efficiency, biomass yield and lactate removal. At 100 microg-Mo l(-1), the maximum rate was 12.0 ml h(-1) with a conversion efficiency of 36.1%, the cell yields were 1.11 g-cell g(-1) -lactate and 2.4 g-cell g(-1)-TOC removed. Further increase of Mo(6+) improved hydrogen production only marginally. Degradation of lactate by R. sphaeroides produced not just hydrogen but also acetate, butyrate, i-valerate, i-caproate, hexanoate and some unidentified organic intermediates, but did not produce propionate and alcohols. Nitrogenase activity, as measured by the acetylene reduction method, had no clear correlation with either Mo(6+) concentration or hydrogen yield.
研究了钼（6+）浓度对以乳酸为唯一碳源的球形红假单胞菌光产氢的影响。结果表明，钼（6+）浓度从无到 1000μg/L 的增加导致了氢气产量、最大产氢率、转化效率、生物量产量和乳酸去除率的增加。在 100μg-Mo/L 时，最大产氢率为 12.0ml/h，转化率为 36.1%，细胞产量为 1.11g-细胞/g-乳酸和 2.4g-细胞/g-TOC 去除。进一步增加钼（6+）对产氢的改善作用不大。球形红假单胞菌降解乳酸不仅产生氢气，还产生乙酸盐、丁酸盐、异戊酸盐、异己酸盐、己酸盐和一些未鉴定的有机中间产物，但不产生丙酸盐和醇。乙炔还原法测定的固氮酶活性与钼（6+）浓度或氢气产量均无明显相关性。
