Residues and Resource Reclamation Centre (R3C), Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, CleanTech One, Singapore, 637141, Singapore.
School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore, 637459, Singapore.
Appl Biochem Biotechnol. 2018 May;185(1):257-269. doi: 10.1007/s12010-017-2655-5. Epub 2017 Nov 10.
Fluorescent and incandescent lighting systems were applied for batch photofermentative hydrogen production by four purple non-sulfur photosynthetic bacteria (PNSB). The hydrogen production efficiency of Rhodopseudomonas palustris, Rhodobacter sphaeroides, Rhodobacter capsulatus, and Rhodospirillum rubrum was evaluated using different carbon sources (acetate, butyrate, lactate, and malate). Incandescent light was found to be more effective for bacteria cell growth and hydrogen production. It was observed that PNSB followed substrate selection criteria for hydrogen production. Only R. palustris was able to produce hydrogen using most carbon sources. Cell density was almost constant, but cell growth rate and hydrogen production were significantly varied under the different lighting systems. The kinetics study suggested that initial substrate concentration had a positive correlation with lag phase duration. Among the PNSB, R. palustris grew faster and had higher hydrogen yields of 1.58, 4.92, and 2.57 mol H/mol using acetate, butyrate, and lactate, respectively. In the integrative approach with dark fermentation effluents rich in organic acids, R. palustris should be enriched in the phototrophic microbial consortium of the continuous hydrogen production system.
荧光灯和白炽灯系统被应用于四种紫色非硫光合细菌（PNSB）的分批光发酵产氢。使用不同的碳源（乙酸盐、丁酸盐、乳酸盐和苹果酸盐）评估了沼泽红假单胞菌、球形红杆菌、荚膜红细菌和沼泽红螺旋菌的产氢效率。白炽灯被发现更有利于细菌细胞生长和产氢。观察到 PNSB 遵循产氢的底物选择标准。只有沼泽红假单胞菌能够使用大多数碳源产氢。细胞密度几乎保持不变，但在不同的照明系统下，细胞生长速率和产氢量有显著差异。动力学研究表明，初始底物浓度与迟滞期持续时间呈正相关。在 PNSB 中，沼泽红假单胞菌生长更快，分别使用乙酸盐、丁酸盐和乳酸盐的产氢量为 1.58、4.92 和 2.57 mol H/mol。在富含有机酸的黑暗发酵废水中的综合方法中，沼泽红假单胞菌应该在连续产氢系统的光营养微生物联合体中得到富集。
