Biochemical Engineering Research Laboratory, Department of Chemical Engineering, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.
Environ Technol. 2011 Aug-Sep;32(11-12):1375-81. doi: 10.1080/09593330.2010.536788.
The bioremediation of marine sediments contaminated with naphthalene and anthracene was studied under anaerobic conditions to investigate the enhancing effect of a biostimulating agent (Tween 80, silicone oil, pig dung and NPK fertilizer) on the rate of degradation. Sediment samples were amended with the biostimulating agent (alone or in combination). The results showed that all the tested agents, applied individually to the sediments, increased the rate of anthracene and naphthalene degradation, with the pig dung having the greatest effect. The biodegradation data were fitted to a pseudo-first-order kinetic model, from which the biodegradation rate constant, as a measure of the enhancement of degradation rate by the biostimulators, was estimated. The rate constant values were consistently higher for the sediments treated with individual stimulators, or a combination of them, than for the untreated sediment. The contaminated sediment treated with the combination of Tween 80 and pig dung exhibited the highest biodegradation rate. The results indicated that the effect of various biostimulating agents, in combination or alone, on enhancing the degradation rate of anthracene and naphthalene can be arranged in the following order: Tween 80 + pig dung > silicone oil + pig dung > Tween 80 + NPK fertilizer > silicone oil + NPK fertilizer > pig dung > NPK fertilizer > Tween 80 > silicone oil. The addition of biostimulators increased the biodegradation potential of the intrinsic microbial populations; thus, these results will contribute to the development of new strategies for in situ bioremediation of anoxic sediments contaminated with polycyclic aromatic hydrocarbons
采用厌氧条件研究了受萘和蒽污染的海洋沉积物的生物修复，以调查生物刺激剂（吐温 80、硅油、猪粪和 NPK 肥料）对降解速率的增强效果。单独或组合添加生物刺激剂对沉积物进行了改良。结果表明，所有测试的试剂单独应用于沉积物时，均能提高蒽和萘的降解速率，其中猪粪的效果最大。将生物降解数据拟合为拟一级动力学模型，从该模型中估算出生物刺激剂增强降解速率的生物降解率常数，作为测量值。与未处理的沉积物相比，用单个刺激剂或它们的组合处理的沉积物的速率常数值始终更高。用吐温 80 和猪粪组合处理的受污染沉积物表现出最高的生物降解速率。结果表明，各种生物刺激剂（单独或组合）对增强蒽和萘降解速率的影响可以按以下顺序排列：吐温 80 + 猪粪>硅油 + 猪粪>吐温 80 + NPK 肥料>硅油 + NPK 肥料>猪粪>NPK 肥料>吐温 80>硅油。生物刺激剂的添加增加了固有微生物种群的生物降解潜力；因此，这些结果将有助于开发受多环芳烃污染的缺氧沉积物原位生物修复的新策略。
