Zhang Zhen, Zheng Guanyu, Lo Irene M C
Department of Civil and Environmental Engineering, The Hong Kong University of Science and Technology, Hong Kong, China.
Environ Sci Pollut Res Int. 2015 Jun;22(11):8296-306. doi: 10.1007/s11356-014-3979-0. Epub 2014 Dec 23.
The effect of microemulsion on the biodegradation of total petroleum hydrocarbons (TPH) in nitrate-induced bioremediation of marine sediment was investigated in this study. It was shown that the microemulsion formed with non-ionic surfactant polyoxyethylene sorbitan monooleate (Tween 80), 1-pentanol, linseed oil, and either deionized water or seawater was stable when subjected to dilution by seawater. Desorption tests revealed that microemulsion was more effective than the Tween 80 solution or the solution containing Tween 80 and 1-pentanol to desorb TPH from marine sediment. In 3 weeks of bioremediation treatment, the injection of microemulsion and NO3 (-) seems to have delayed the autotrophic denitrification between NO3 (-) and acid volatile sulfide (AVS) in sediment compared to the control with NO3 (-) injection alone. However, after 6 weeks of treatment, the delaying effect of microemulsion on the autotrophic denitrification process was no longer observed. In the meantime, the four injections of microemulsion and NO3 (-) resulted in as high as 29.73 % of TPH degradation efficiency, higher than that of two injections of microemulsion and NO3 (-) or that of four or two injections of NO3 (-) alone. These results suggest that microemulsion can be potentially applied to enhance TPH degradation in the nitrate-induced bioremediation of marine sediment.
本研究考察了微乳液对硝酸盐诱导的海洋沉积物生物修复中总石油烃（TPH）生物降解的影响。结果表明，由非离子表面活性剂聚氧乙烯山梨醇酐单油酸酯（吐温80）、1-戊醇、亚麻籽油以及去离子水或海水形成的微乳液在受到海水稀释时是稳定的。解吸试验表明，微乳液从海洋沉积物中解吸TPH的效果比吐温80溶液或含有吐温80和1-戊醇的溶液更有效。在3周的生物修复处理中，与仅注入硝酸盐（NO3 (-)）的对照组相比，注入微乳液和NO3 (-)似乎延迟了沉积物中NO3 (-)与酸挥发性硫化物（AVS）之间的自养反硝化作用。然而，在处理6周后，不再观察到微乳液对自养反硝化过程的延迟作用。同时，四次注入微乳液和NO3 (-)导致TPH降解效率高达29.73%，高于两次注入微乳液和NO3 (-)或单独四次或两次注入NO3 (-)的情况。这些结果表明，微乳液在硝酸盐诱导的海洋沉积物生物修复中具有增强TPH降解的潜在应用价值。
