Zaidi M, Seth R, Girgis S I, Self C H
Department of Cellular and Molecular Sciences, St. George's Hospital Medical School, Tooting, London, U.K.
Clin Chem. 1990 Jul;36(7):1288-94.
Calcitonin gene-related peptide (CGRP), a potent endogenous circulating vasodilator, is produced by the alternative splicing of the calcitonin/CGRP gene and is expressed mainly in neural and cardiovascular tissues. We recently reported a highly sensitive radioimmunoassay of CGRP, based on an antiserum recognizing the C-terminus of the molecule. We have also found that circulating immunoreactive CGRP is heterogeneous; thus we are unable to measure selectively the intact molecule with our one-site competitive approach. We therefore attempted to construct a two-site immunometric assay involving two antibodies, one that detects the C-terminus and another that recognizes the midregion of the molecule. To enhance assay sensitivity, we applied a colorimetric amplification system to this assay. This rapid, robust, and reproducible assay provides more nearly accurate estimates of circulating CGRP and offers a sensitive and more specific alternative to the radioimmunoassay, with advantages in speed, simplicity, and convenience.
降钙素基因相关肽（CGRP）是一种强大的内源性循环血管舒张剂，由降钙素/CGRP基因的可变剪接产生，主要在神经和心血管组织中表达。我们最近报道了一种基于识别该分子C末端的抗血清的高灵敏度CGRP放射免疫测定法。我们还发现循环中的免疫反应性CGRP具有异质性；因此，我们无法用我们的单位点竞争方法选择性地测量完整分子。因此，我们试图构建一种双位点免疫测定法，使用两种抗体，一种检测C末端，另一种识别分子的中间区域。为了提高测定灵敏度，我们将比色扩增系统应用于该测定法。这种快速、稳健且可重复的测定法能更准确地估计循环中的CGRP，并为放射免疫测定法提供了一种灵敏且更特异的替代方法，在速度、简便性和便利性方面具有优势。
