Zaidi M, Girgis S I, MacIntyre I
Department of Chemical Pathology, Royal Postgraduate Medical School, Hammersmith Hospital, London, U.K.
Clin Chem. 1988 Apr;34(4):655-60.
The calcitonin genes encode a small family of peptides: the circulating hormone calcitonin; its flanking peptide, katacalcin; and a third novel peptide, calcitonin gene-related peptide (CGRP). CGRP is a potent vasodilator and a major circulating product from the calcitonin genes; it may be a physiologically important regulator of blood flow in humans. High concentrations of circulating CGRP are found in medullary thyroid carcinoma. We report the development and validation of a highly sensitive (detection limit 500 amol per tube) radioimmunoassay of CGRP involving a high-affinity antibody directed against the carboxyl terminus of the molecule and a highly pure tracer. The assay is precise, robust, and reproducible, and is therefore a potentially useful analytical method for studying the normal and abnormal physiology of this peptide.
循环激素降钙素；其侧翼肽，降钙素原；以及第三种新型肽，降钙素基因相关肽（CGRP）。CGRP是一种强效血管舒张剂，是降钙素基因的主要循环产物；它可能是人体血流的重要生理调节因子。甲状腺髓样癌患者体内循环CGRP浓度较高。我们报告了一种高灵敏度（每管检测限500 amol）的CGRP放射免疫分析方法的开发与验证，该方法涉及一种针对该分子羧基末端的高亲和力抗体和一种高纯度示踪剂。该分析方法精确、稳健且可重复，因此是研究该肽正常和异常生理学的一种潜在有用的分析方法。
