Hanley M R, Cheung W T, Hawkins P, Poyner D, Benton H P, Blair L, Jackson T R, Goedert M
MRC Molecular Neurobiology Unit, MRC Centre, Cambridge, UK.
Ciba Found Symp. 1990;150:23-38; discussion 38-46. doi: 10.1002/9780470513927.ch3.
The human mas oncogene, which renders transfected NIH/3T3 cells tumorigenic, was identified as a subtype of angiotensin receptor by transient expression in Xenopus oocytes and stable expression in the mammalian neuronal cell line, NG115-401L. The mas receptor preferentially recognizes angiotensin III, and is expressed at high levels in brain. The mas/angiotensin receptor functions through the breakdown of inositol lipids and can drive DNA synthesis, unlike another inositol-linked peptide receptor, that for bradykinin. Comparative analysis of several early biochemical events elicited by either angiotensin or bradykinin stimulation of mas-transfected cells has not indicated a specific difference correlated with mitogenic activity. In particular, the inositol lipid kinase, phosphatidylinositol-3-kinase, thought to be involved in the mitogenic mechanism of platelet-derived growth factor receptors, is unaffected by activation of mas. These results have shown that a proto-oncogene encodes a neural peptide receptor, indicating that peptide receptors may be involved in differentiation and proliferation processes, as are other identified proto-oncogenes.
人类mas癌基因可使转染的NIH/3T3细胞具有致瘤性，通过在非洲爪蟾卵母细胞中瞬时表达以及在哺乳动物神经细胞系NG115 - 401L中稳定表达，被鉴定为血管紧张素受体的一种亚型。mas受体优先识别血管紧张素III，且在脑中高水平表达。与另一种肌醇连接肽受体（缓激肽受体）不同，mas/血管紧张素受体通过肌醇脂质的分解发挥作用，并能驱动DNA合成。对血管紧张素或缓激肽刺激mas转染细胞引发的几个早期生化事件的比较分析，未显示出与促有丝分裂活性相关的特定差异。特别是，被认为参与血小板衍生生长因子受体促有丝分裂机制的肌醇脂质激酶——磷脂酰肌醇-3-激酶，不受mas激活的影响。这些结果表明，一个原癌基因编码一种神经肽受体，这表明肽受体可能如其他已鉴定的原癌基因一样，参与分化和增殖过程。
