Univ. de Bordeaux, Unité sous Contrat Mycoplasmal and chlamydial infections in humans.
J Infect Dis. 2011 Dec 1;204(11):1796-805. doi: 10.1093/infdis/jir630. Epub 2011 Oct 4.
Mycoplasma hominis, a human urogenital pathogen, is involved in genital and extragenital infections and arthritis, particularly in immunocompromised patients. The interleukin (IL) 23/T helper (Th) 17 axis is associated with inflammatory and autoimmune diseases. The aim of this study was to assess the IL-23 response to M. hominis in human dendritic cells (DCs) and the CD4(+) T-cell differentiation in response to M. hominis-infected DCs.
Human monocyte-derived DCs were cultured with phosphate-buffered saline, lipopolysaccharide, or M. hominis PG21. Cocultures with heterologous T cells were performed. Extracts from M. hominis were separated and incubated with DCs. Isolates from different clinical syndromes were tested.
M. hominis induced the maturation of human DCs with predominant IL-23 secretion in a Toll-like receptor 2-dependent manner. The in vitro immunomodulatory capacity of M. hominis was contained in a lipoprotein-enriched fraction from the mycoplasma. M. hominis-activated DCs induced IL-17-producing CD4(+) T cells. Interestingly, clinical isolates differed in their ability to promote IL-23 secretion by DCs.
Taken together, our findings demonstrate a major role for the IL-23/Th17 axis in the defense against M. hominis and indicate a potential role for these bacteria in inflammatory and autoimmune diseases.
人型支原体是一种人类泌尿生殖道病原体，与生殖器和生殖器外感染和关节炎有关，尤其是在免疫功能低下的患者中。白细胞介素 (IL) 23/T 辅助 (Th) 17 轴与炎症和自身免疫性疾病有关。本研究旨在评估人型支原体对人树突状细胞 (DC) 的 IL-23 反应以及对人型支原体感染的 DC 反应的 CD4(+) T 细胞分化。
培养人单核细胞来源的 DCs，分别用磷酸盐缓冲液、脂多糖或人型支原体 PG21 刺激。进行异源 T 细胞共培养。用 DC 分离并孵育人型支原体提取物。测试来自不同临床综合征的分离株。
人型支原体以 Toll 样受体 2 依赖性方式诱导人 DCs 的成熟，主要分泌 IL-23。人型支原体的体外免疫调节能力包含在该支原体富含脂蛋白的部分中。人型支原体激活的 DC 诱导产生 IL-17 的 CD4(+) T 细胞。有趣的是，临床分离株在促进 DC 分泌 IL-23 的能力上存在差异。
总之，我们的研究结果表明 IL-23/Th17 轴在抵抗人型支原体方面发挥着重要作用，并表明这些细菌在炎症和自身免疫性疾病中可能发挥作用。
