Tuxun Tuerhongjiang, Ma Hai-Zhang, Apaer Shadike, Zhang Heng, Aierken Amina, Li Yu-Peng, Lin Ren-Yong, Zhao Jin-Ming, Zhang Jin-Hui, Wen Hao
State Key Laboratory Incubation Base of Xinjiang Major Diseases Research and Xinjiang Key Laboratory of Echinococcosis, First Affiliated Hospital of Xinjiang Medical University, Ürümqi 830054, China ; Department of Liver and Laparoscopic Surgery, Digestive and Vascular Centre, First Affiliated Hospital of Xinjiang Medical University, Ürümqi 830054, China.
State Key Laboratory Incubation Base of Xinjiang Major Diseases Research and Xinjiang Key Laboratory of Echinococcosis, First Affiliated Hospital of Xinjiang Medical University, Ürümqi 830054, China ; Department of General Surgery, Qilu Hospital, Shandong 250000, China.
Mediators Inflamm. 2015;2015:632760. doi: 10.1155/2015/632760. Epub 2015 Nov 9.
Several studies have demonstrated the important role of Toll-like receptors in various parasitic infections. This study aims to explore expression of Toll-like receptors (TLRs) and related cytokines in patients with human cystic echinococcosis (CE) and alveolar echinococcosis (AE). 78 subjects including AE group (N = 28), CE group (N = 22), and healthy controls (HC, N = 28) were enrolled in this study. The mRNA expression levels of TLR2 and TLR4 in blood and hepatic tissue and plasma levels related cytokines were detected by using ELISA. Median levels of TLR2 mRNA in AE and CE groups were significantly elevated as compared with that in healthy control group. Median levels of TLR4 expression were increased in AE and CE. Plasma concentration levels of IL-5, IL-6, and IL-10 were slightly increased in AE and CE groups compared with those in HC group with no statistical differences (p > 0.05). The IL-23 concentration levels were significantly higher in AE and CE groups than that in HC subjects with statistical significance. The increased expression of TLR2 and IL-23 might play a potential role in modulating tissue infiltrative growth of the parasite and its persistence in the human host.
多项研究已证实Toll样受体在各种寄生虫感染中发挥的重要作用。本研究旨在探讨人类囊型棘球蚴病（CE）和泡型棘球蚴病（AE）患者中Toll样受体（TLRs）及相关细胞因子的表达情况。本研究纳入了78名受试者，包括AE组（N = 28）、CE组（N = 22）和健康对照组（HC，N = 28）。采用ELISA法检测血液和肝组织中TLR2和TLR4的mRNA表达水平以及血浆中相关细胞因子的水平。与健康对照组相比，AE组和CE组中TLR2 mRNA的中位数水平显著升高。AE组和CE组中TLR4的表达中位数水平升高。与HC组相比，AE组和CE组中IL-5、IL-6和IL-10的血浆浓度水平略有升高，但无统计学差异（p > 0.05）。AE组和CE组中IL-23的浓度水平显著高于HC组受试者，具有统计学意义。TLR2和IL-23表达的增加可能在调节寄生虫在组织中的浸润生长及其在人类宿主中的持续存在方面发挥潜在作用。
