L-丙氨酰-谷氨酰胺预处理可减轻大鼠肝脏缺血再灌注损伤。

Preconditioning with L-alanyl-glutamine reduces hepatic ischemia-reperfusion injury in rats.

作者信息

Araújo Júnior Raimundo José Cunha, Silva Júnior Raimundo Gerônimo da, Vasconcelos Marcelo Pinho Pessoa de, Guimarães Sérgio Botelho, Vasconcelos Paulo Roberto Leitão de, Garcia José Huygens Parente

机构信息

Department of Surgery, Federal University of Ceara, Fortaleza-CE, Brazil.

出版信息

Acta Cir Bras. 2011;26 Suppl 1:8-13. doi: 10.1590/s0102-86502011000700003.

DOI:10.1590/s0102-86502011000700003

PMID:21971650

Abstract

PURPOSE

To evaluate the effects of pre-conditioning with L-alanyl-glutamine (L-Ala-Gln) in rats subjected to total hepatic ischemia.

METHODS

Thirty Wistar rats, average weight 300g, were randomly assigned to 3 groups (n=10): G-1--saline, G-2--L-Ala-Gln, G-3--control (Sham). G-1 and G-3 groups were treated with saline 2.0 ml or L-Ala-Gln (0.75mg/Kg) intraperitoneally (ip) respectively, 2 hours before laparotomy. Anesthetized rats were subjected to laparotomy and total hepatic ischemia (30 minutes) induced by by clamping of portal triad. Control group underwent peritoneal puncture, two hours before the sham operation (laparotomy only). At the end of ischemia (G1 and G2), the liver was reperfused for 60 minutes. Following reperfusion blood samples were collected for evaluation of alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) levels. Liver (medium lobe) was removed for immunohistochemistry study with antibody for Caspase-3.

RESULTS

It was found a significant decrease (p<0.05) of ALT levels (270.6 +40.8 vs 83.3 +5.5 - p <0.05), LDH (2079.0 +262.4 vs. 206.6 +16.2 - p <0.05) and Caspase-3 expression (6.72 +1.35 vs. 2.19 +1.14, p <0.05) in rats subjected to I / R, comparing the group treated with L-Ala -Gln with G-2. Also, the ALT level was significantly lower (P<0.05) in G-1 and G-2 groups than in G-3 (control group).

CONCLUSION

L-Ala-Gln preconditioning in rats submitted to hepatic I/R significantly reduces ALT, LDH and Caspase-3 expression, suggesting hepatic protection.

摘要

目的

评估L-丙氨酰-谷氨酰胺（L-Ala-Gln）预处理对全肝缺血大鼠的影响。

方法

30只平均体重300g的Wistar大鼠随机分为3组（n = 10）：G-1组——生理盐水组，G-2组——L-Ala-Gln组，G-3组——对照组（假手术组）。G-1组和G-3组在剖腹术前2小时分别腹腔注射2.0 ml生理盐水或L-Ala-Gln（0.75mg/Kg）。麻醉后的大鼠进行剖腹手术，并通过夹闭门静脉三联诱导全肝缺血（30分钟）。对照组在假手术（仅剖腹术）前2小时进行腹腔穿刺。缺血结束时（G1组和G2组），肝脏再灌注60分钟。再灌注后采集血样以评估丙氨酸转氨酶（ALT）和乳酸脱氢酶（LDH）水平。取出肝脏（中叶）用Caspase-3抗体进行免疫组化研究。

结果

与G-2组相比，L-Ala -Gln处理组的大鼠在缺血/再灌注后ALT水平（270.6 +40.8 vs 83.3 +5.5 - p <0.05）、LDH（2079.0 +262.4 vs. 206.6 +16.2 - p <0.05）和Caspase-3表达（6.72 +1.35 vs. 2.19 +1.14，p <0.05）显著降低。此外，G-1组和G-2组的ALT水平显著低于G-3组（对照组）（P<0.05）。