General Hospital of Guangzhou Military Command of PLA, Guangzhou, Guangdong 510515, PR China.
Mol Med Rep. 2012 Jan;5(1):48-53. doi: 10.3892/mmr.2011.612. Epub 2011 Oct 3.
T lymphoma invasion and metastasis 1 (Tiam1) is a guanine nucleotide exchange factor (GNEF) family member, and is considered to be involved in many important cellular processes and oncogenesis. In this study, we investigated Tiam1 expression differences between normal tissue and malignant tissue using tissue microarray (TMA), and further studied the Tiam1 mRNA and protein level in 9 hepatoma lines. Forty-nine tumor tissue and 47 normal tissue samples were detected via TMA by immunohistochemistry with polyclonal antibody. Tiam1 expression in 9 human hepatoma cell lines, namely Huh-7, HepG2, Hep3B, SMMC-7721, QGy-7701, QGy-7703, BEL-7402, BEL-7404 and BEL-7405, and 1 normal primary human hepatocyte, HL-7702, was compared by means of fluorescence quantitative PCR, immunocytochemistry assay and Western blotting. We found that Tiam1 was significantly expressed in various malignancies. Tiam1 mRNA and protein levels were significantly elevated in the 9 human hepatoma cell lines compared to the normal primary human hepatocyte. Our results suggest that Tiam1 overexpression in malignant neoplasms could be a novel effective supplementary biomarker for tumors, including hepatocellular carcinoma.
T 淋巴瘤侵袭和转移 1（Tiam1）是鸟嘌呤核苷酸交换因子（GNEF）家族的一员，被认为参与许多重要的细胞过程和肿瘤发生。在这项研究中，我们使用组织微阵列（TMA）研究了正常组织和恶性组织之间 Tiam1 的表达差异，并进一步研究了 9 个人肝癌系中的 Tiam1 mRNA 和蛋白水平。通过免疫组织化学用多克隆抗体对 49 个肿瘤组织和 47 个正常组织样本进行了 TMA 检测。通过荧光定量 PCR、免疫细胞化学检测和 Western blot 比较了 9 个人肝癌细胞系（Huh-7、HepG2、Hep3B、SMMC-7721、QGy-7701、QGy-7703、BEL-7402、BEL-7404 和 BEL-7405）和 1 个正常原代人肝细胞（HL-7702）中 Tiam1 的表达。结果发现，Tiam1 在各种恶性肿瘤中均有明显表达。与正常原代人肝细胞相比，9 个人肝癌细胞系中 Tiam1 mRNA 和蛋白水平显著升高。我们的结果表明，恶性肿瘤中 Tiam1 的过度表达可能是包括肝细胞癌在内的肿瘤的一种新的有效补充标志物。
