Orthopaedic Research Laboratory, Aarhus University Hospital, Nørrebrogade 44, Build. 1A, 1. Floor, 8000 Aarhus C, Denmark.
Knee Surg Sports Traumatol Arthrosc. 2012 Jun;20(6):1192-204. doi: 10.1007/s00167-011-1692-9. Epub 2011 Oct 5.
To develop a nano-structured porous polycaprolactone (NSP-PCL) scaffold and compare the articular cartilage repair potential with that of a commercially available collagen type I/III (Chondro-Gide) scaffold.
By combining rapid prototyping and thermally induced phase separation, the NSP-PCL scaffold was produced for matrix-assisted autologous chondrocyte implantation. Lyophilizing a water-dioxane-PCL solution created micro and nano-pores. In vitro: The scaffolds were seeded with rabbit chondrocytes and cultured in hypoxia for 6 days. qRT-PCR was performed using primers for sox9, aggrecan, collagen type 1 and 2. In vivo: 15 New Zealand White Rabbits received bilateral osteochondral defects in the femoral intercondylar grooves. Autologous chondrocytes were harvested 4 weeks prior to surgery. There were 3 treatment groups: (1) NSP-PCL scaffold without cells. (2) The Chondro-Gide scaffold with autologous chondrocytes and (3) NSP-PCL scaffold with autologous chondrocytes. Observation period was 13 weeks. Histological evaluation was made using the O'Driscoll score.
In vitro: The expressions of sox9 and aggrecan were higher in the NSP-PCL scaffold, while expression of collagen 1 was lower compared to the Chondro-Gide scaffold. In vivo: Both NSP-PCL scaffolds with and without cells scored significantly higher than the Chondro-Gide scaffold when looking at the structural integrity and the surface regularity of the repair tissue. No differences were found between the NSP-PCL scaffold with and without cells.
The NSP-PCL scaffold demonstrated higher in vitro expression of chondrogenic markers and had higher in vivo histological scores compared to the Chondro-Gide scaffold. The improved chondrocytic differentiation can potentially produce more hyaline cartilage during clinical cartilage repair. It appears to be a suitable cell-free implant for hyaline cartilage repair and could provide a less costly and more effective treatment option than the Chondro-Gide scaffold with cells.
开发一种纳米结构多孔聚己内酯（NSP-PCL）支架，并将其与市售的 I/III 型胶原（Chondro-Gide）支架的关节软骨修复潜力进行比较。
通过快速成型技术和热致相分离法，制备用于基质辅助自体软骨细胞植入的 NSP-PCL 支架。将水-二氧六环-PCL 溶液冻干，形成微纳米孔。体外：将支架与兔软骨细胞共培养，在低氧条件下培养 6 天。使用 sox9、聚集蛋白聚糖、胶原 1 和 2 的引物进行 qRT-PCR。体内：15 只新西兰白兔双侧股骨髁间窝骨软骨缺损。在手术前 4 周收获自体软骨细胞。有 3 个治疗组：（1）无细胞的 NSP-PCL 支架。（2）含自体软骨细胞的 Chondro-Gide 支架和（3）含自体软骨细胞的 NSP-PCL 支架。观察期为 13 周。采用 O'Driscoll 评分进行组织学评估。
体外：NSP-PCL 支架的 sox9 和聚集蛋白聚糖表达较高，而与 Chondro-Gide 支架相比，胶原 1 的表达较低。体内：无论是否有细胞，NSP-PCL 支架的结构完整性和修复组织表面规则性评分均明显高于 Chondro-Gide 支架。NSP-PCL 支架有无细胞的评分之间无差异。
与 Chondro-Gide 支架相比，NSP-PCL 支架在体外表现出更高的软骨生成标志物表达，在体内具有更高的组织学评分。改善的软骨细胞分化在临床软骨修复过程中可能产生更多的透明软骨。它似乎是一种适合透明软骨修复的无细胞植入物，并且可能比含细胞的 Chondro-Gide 支架提供更经济有效的治疗选择。
