Department of Gastroenterology, Affiliated Hospital, Nantong University, 226001 Nantong, People's Republic of China.
Med Oncol. 2012 Sep;29(3):1748-57. doi: 10.1007/s12032-011-0067-x. Epub 2011 Oct 5.
The molecular mechanisms leading to gastric carcinogenesis still remain unclear. Recently, several studies demonstrated that over-expression of guanylyl cyclase C (GCC) has been detected in intestinal-type gastric cancer (GC) and precursor lesions. Our objective was to explore the expression levels of GCC and endogenous ligands guanylin (GN) and uroguanylin (UGN) and the correlation between Helicobacter pylori (H. pylori) and GCC, GN, and UGN expressions in patients at different stages from normal mucosa to superficial gastritis, atrophic gastritis, intestinal metaplasia (IM), dysplasia, and finally adenocarcinoma. The expression of GCC and GN was absent in the distal normal gastric tissues and superficial gastritis in all cases, whereas they were measured in IM, dysplasia, and GC. The expression of GCC and GN was closely related to intestinal-type GC. From superficial gastritis to gastric carcinomas, the H. pylori positive rate was 19.7, 33.3, 69.6, 80.0, and 82.1%, respectively. The positive correlation was found between GCC and GN in IM, dysplasia, and GC. Also, the positive correlation was found between GCC, GN, and H. pylori infection in them. These results demonstrate that the detection of GCC and GN will be beneficial to diagnosis human gastric carcinoma and precancerous lesions. Ectopic expression of GCC and GN in human gastric mucosa and H. pylori infection may play an important role in the carcinogenesis of the intestinal-type GC.
导致胃癌发生的分子机制仍不清楚。最近，几项研究表明，鸟苷酸环化酶 C（GCC）在肠型胃癌（GC）及其前体病变中过度表达。我们的目的是探讨 GCC 及其内源性配体鸟苷素（GN）和尿鸟苷素（UGN）在从正常黏膜到浅表性胃炎、萎缩性胃炎、肠上皮化生（IM）、异型增生到最终腺癌的不同阶段患者中的表达水平，以及 H. pylori（H. pylori）与 GCC、GN 和 UGN 表达之间的相关性。在所有情况下，远端正常胃组织和浅表性胃炎中均未检测到 GCC 和 GN 的表达，而在 IM、异型增生和 GC 中则有测量到。GCC 和 GN 的表达与肠型 GC 密切相关。从浅表性胃炎到胃癌，H. pylori 的阳性率分别为 19.7%、33.3%、69.6%、80.0%和 82.1%。在 IM、异型增生和 GC 中发现 GCC 和 GN 之间存在正相关。此外，在这些病变中还发现了 GCC、GN 和 H. pylori 感染之间的正相关。这些结果表明，检测 GCC 和 GN 将有助于诊断人类胃癌和癌前病变。GCC 和 GN 在人胃黏膜中的异位表达以及 H. pylori 感染可能在肠型 GC 的发生中发挥重要作用。
