College of Animal Science and Technology, Shaan'xi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, YangLing, Shaan'xi, 712100, PR China.
Yeast. 2011 Nov;28(11):795-8. doi: 10.1002/yea.1905. Epub 2011 Oct 4.
A new method for protein extraction from yeast Saccharomyces cerevisiae cells is described. This method involves the use of LiAc and NaOH to enhance the permeability of yeast cell wall prior to protein extraction with SDS-PAGE sample buffer. It was safe and efficient compared to other methods reported so far in the literature. The proteins extracted with this new method retained their immunoreactive properties and are suitable for most applications in molecular biology studies.
一种从酵母 Saccharomyces cerevisiae 细胞中提取蛋白质的新方法被描述。该方法涉及使用 LiAc 和 NaOH 来增强酵母细胞壁的通透性，然后用 SDS-PAGE 样品缓冲液提取蛋白质。与迄今为止文献中报道的其他方法相比，该方法既安全又高效。用这种新方法提取的蛋白质保留了其免疫反应性，适用于分子生物学研究中的大多数应用。
