Division of Microbiology, College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Biotechnol Lett. 2012 Jan;34(1):153-8. doi: 10.1007/s10529-011-0754-x. Epub 2011 Oct 5.
Racemic 2-nitro-1-phenylethanol was resolved by via enantioselective transesterification catalyzed by Burkholderia cepacia lipase. The reaction afforded excellent E values (E > 200) and enantioselectivity (up to >99% enantiomeric excesses [ee]) of both remaining substrates and acetylated product. Moreover, the lipase displayed high enantioselectivity in the resolution of additional 2-nitroalcohols (E up to >200). This method provides an efficient alternative for obtaining enantiopure 2-nitroalcohols.
(R)-2-硝基-1-苯乙醇通过假单胞菌属脂酶催化的对映选择性转酯化拆分。该反应对剩余底物和乙酰化产物均表现出优异的对映体过量值 (E > 200) 和对映选择性 (最高 >99%对映体过量)。此外，该脂肪酶在拆分其他 2-硝基醇时也表现出高对映选择性 (E 最高 >200)。该方法为获得手性纯 2-硝基醇提供了有效的替代方法。
