Faculty of Chemistry, University of San Luìs Potosí, San Luis Potosí, México.
Int J Phytoremediation. 2011 Jul;13(6):538-51. doi: 10.1080/15226511003671379.
The ability of in vitro roots cultures of Typha latifolia and Scirpus americanus to remove metals was studied. Roots were cultivated on Murashige-Skoog medium with 15 microg L(-1) Cr 11, 60 microg L(-1) Pb II or 1.8 mg L(-1) Mn II. Adsorbed metal to root surface was removed by washing with 0.042% HNO3. T. latifolia roots were able to uptake 68.8 microg Pb g(-1), 22.1 microg Cr g(-1) and 1680 microg Mn g(-1), while the S. americanus roots removed 148.3 microg Pb g(-1), 40.7 microg Cr g(-1) and 4037 microg Mn g(-1). About 80-90% of Pb and Cr were absorbed in both cultures. On the contrary, the Mn removal was due mainly to an adsorption process (82-86%). In comparison to the T. latifolia cultures, S. americanus cultures were twofold more efficient to remove Pb and Cr, and threefold more efficient to remove Mn. Both plant species capture metals in the following order: Cr >Pb >Mn. This investigation confirms that in vitro roots cultures could be an alternative as a phytoremediation approach for contaminated water with heavy metals.
研究了香蒲和蔺草的离体根培养物去除金属的能力。将根在含有 15μg L(-1)Cr 11 、60μg L(-1)Pb II 或 1.8mg L(-1)Mn II 的 Murashige-Skoog 培养基上培养。用 0.042% HNO3 冲洗去除吸附在根表面的金属。香蒲根可吸收 68.8μg Pb g(-1)、22.1μg Cr g(-1)和 1680μg Mn g(-1)，而蔺草根可吸收 148.3μg Pb g(-1)、40.7μg Cr g(-1)和 4037μg Mn g(-1)。两种培养物中约 80-90%的 Pb 和 Cr 被吸收。相反，Mn 的去除主要是由于吸附过程（82-86%）。与香蒲培养物相比，蔺草培养物去除 Pb 和 Cr 的效率高两倍，去除 Mn 的效率高三倍。两种植物对金属的摄取顺序为：Cr >Pb >Mn。本研究证实，离体根培养物可以作为一种植物修复受重金属污染水的替代方法。
