Hernández-Piedra Guadalupe, Ruiz-Carrera Violeta, Sánchez Alberto J, Escalante-Espinosa Erika, Calva-Calva Graciano
Programa de Doctorado en Ecología y Manejo de Sistemas Tropicales, Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa-Cárdenas Km. 0.5 S/N Entronque a Bosques de Saloya, Villahermosa C.P. 86150, Tabasco, Mexico.
Diagnóstico y Manejo de Humedales Tropicales, Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa-Cárdenas Km. 0.5 S/N Entronque a Bosques de Saloya, Villahermosa C.P. 86150, Tabasco, Mexico.
Life (Basel). 2025 Jan 17;15(1):121. doi: 10.3390/life15010121.
The spatial-temporal dynamics of an in vitro radicular system of for the development of rhizofiltration technologies, with the potential for use as a phytotreatment of eutrophicated water, were studied for the first time in the roots of seedlings and in rhizotron systems. The effect of indole-3-acetic acid (AIA) in combination with kinetin (CIN) or 6-benzylaminopurine (BAP) on seedlings cultivated in the light and dark in three radicular systems and in a rhizotrophic regime for the screening of dynamic rhizogenic lines, by weekly allometric measurements of the length and number of roots, were studied. Inhibition of the elongation and branching velocities of roots by BAP and light was observed but CIN increased elongation and branching. In rhizotrons cultivated in light and dark conditions with different AIA/CIN ratios, isolated root explants remained inactive; however, roots attached to a meristematic base presented a significant increase in growth development, with values comparable to those of roots attached to seedlings cultivated in light without hormones. The results revealed that six adventitious rhizogenic root lines with basal meristems have the potential for use in a wide range of environmental and innovative applications in phytotreatment technologies involving eutrophicated water.
首次在幼苗根系和根箱系统中研究了用于根际过滤技术开发的体外根系系统的时空动态，该技术具有用于富营养化水植物处理的潜力。通过每周对根的长度和数量进行异速生长测量，研究了吲哚 - 3 - 乙酸（AIA）与激动素（CIN）或6 - 苄基腺嘌呤（BAP）组合对在三种根系系统中于光照和黑暗条件下培养的幼苗以及在根际营养条件下筛选动态生根系的影响。观察到BAP和光照对根的伸长和分支速度有抑制作用，但CIN增加了伸长和分支。在不同AIA/CIN比例的光照和黑暗条件下培养的根箱中，分离的根外植体保持不活跃；然而，附着在分生组织基部的根生长发育显著增加，其值与在无激素光照条件下培养的附着在幼苗上的根相当。结果表明，具有基部分生组织的六条不定生根系有潜力用于涉及富营养化水的植物处理技术中的广泛环境和创新应用。
