[评估不同氧疗对急性氮气窒息大鼠的影响]
[Evaluating the effects of different oxygen therapies on the rats with acute nitrogen asphyxia].
作者信息
Xue Chang-jiang, Xia Yu-jing, Niu Ying-mei, Hao Feng-tong, Zhou Shuo, Lu Qing-sheng, Liu Jian-zhong, Zhang Peng
机构信息
Department of Occupational Diseases and Toxication, Chaoyang Hospital Affiliated to the Capital University of Medical Science, Beijing 100020, China.
出版信息
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2011 May;29(5):361-3.
OBJECTIVE
To Evaluate the effects of different oxygen therapies on the rats with acute nitrogen asphyxia and to study the best oxygen therapic protocol for patients with acute nitrogen asphyxia on the spot.
METHODS
Sixty healthy male Wistar rats were divided into 5 groups: control, exposure to nitrogen, 33% oxygen treatment, 50% oxygen treatment and hyperbaric oxygen treatment groups. The behavioral performance, arterial oxygen pressure (PO2), carbon dioxide partial pressure (PCO2) and oxygen saturation (SPO2), biochemical changes in liver and kidney function and myocardial enzymes in 5 groups were measured.
RESULTS
The rats exposed to nitrogen firstly were excited then inactive symptoms, but consciousness was recovered after oxygen therapy. The PO2 and SPO2 in nitrogen exposure group were (79.67 +/- 9.12) and (94.92 +/- 2.78) mm Hg, respectively, which were significantly lower than those in control group (P<0.01). The PO2 and SPO2 of 3 oxygen treatment groups were (94.75 +/- 7.24), (94.92 +/- 8.98), (104.58 +/- 7.12)mm Hg and (97.17 +/- 0.83), (96.92 +/- 1.16), (97.42 +/- 0.67)mm Hg, respectively, which were significantly higher than those in nitrogen exposure group (P<0.05). The PO2 in hyperbaric oxygen treatment group was significantly higher than those in other 2 oxygen treatment groups (P<0.05). The SPO2 in hyperbaric oxygen treatment group was (51.42 +/- 6.60) mm Hg which was significantly higher than that [(44.58 +/- 3.42)mm Hg] in 50% oxygen treatment groups (P< 0.05). AST [(270.50 +/- 49.05 )U/L], ALT [(122.67 +/- 55.44 )U/L], BUN [(7.31 +/- 0.93 )mmol/L], Cr[(28.32 +/- 4.35) micromol/L], CK [(1808.42 +/- 582.05)U/L] and CtnI [(22.52 +/- 14.29 )ng/ml] in nitrogen exposure group were significantly higher than those in control group (P<0.05). AST [(165.25 +/- 30.87) U/L], HBDH [(350.83 +/- 103.00)U/L] and CtnI [(11.23 +/- 5.38) ng/ml] in hyperbaric oxygen treatment group were significantly lower than those in other 2 oxygen treatment groups (P<0.05).
CONCLUSION
Timely and effective oxygen therapy can significantly increase arterial pressure of oxygen and oxygen saturation in the rats with acute nitrogen asphyxia, and can improve liver function and cardiac damage. The hyperbaric oxygen chamber can significantly increase the therapeutic effects on rats with acute nitrogen asphyxiation.
目的
评估不同氧疗对急性氮气窒息大鼠的影响，探讨急性氮气窒息患者现场最佳氧疗方案。
方法
将60只健康雄性Wistar大鼠分为5组：对照组、氮气暴露组、33%氧疗组、50%氧疗组和高压氧疗组。测定5组大鼠的行为表现、动脉血氧分压（PO2）、二氧化碳分压（PCO2）和血氧饱和度（SPO2），以及肝肾功能和心肌酶的生化变化。
结果
首先暴露于氮气的大鼠出现兴奋后转为活动减少症状，但氧疗后意识恢复。氮气暴露组的PO2和SPO2分别为（79.67±9.12）和（94.92±2.78）mmHg，显著低于对照组（P<0.01）。3个氧疗组的PO2和SPO2分别为（94.75±7.24）、（94.92±8.98）、（104.58±7.12）mmHg和（97.17±0.83）、（96.92±1.16）、（97.42±0.67）mmHg，显著高于氮气暴露组（P<0.05）。高压氧疗组的PO2显著高于其他2个氧疗组（P<0.05）。高压氧疗组的SPO2为（51.42±6.60）mmHg，显著高于50%氧疗组的[（44.58±3.42）mmHg]（P<0.05）。氮气暴露组的谷草转氨酶（AST）[（270.50±49.05）U/L]、谷丙转氨酶（ALT）[（122.67±55.44）U/L]、尿素氮（BUN）[（7.31±0.93）mmol/L]、肌酐（Cr）[（28.32±4.35）μmol/L]、肌酸激酶（CK）[（1808.42±582.05）U/L]和肌钙蛋白I（CtnI）[（22.52±14.29）ng/ml]显著高于对照组（P<0.05）。高压氧疗组的AST[（165.25±30.87）U/L]、α-羟丁酸脱氢酶（HBDH）[（350.83±103.00）U/L]和CtnI[（11.23±5.38）ng/ml]显著低于其他2个氧疗组（P<0.05）。
结论
及时有效的氧疗可显著提高急性氮气窒息大鼠的动脉血氧分压和血氧饱和度，并可改善肝功能和心脏损伤。高压氧舱对急性氮气窒息大鼠的治疗效果显著增强。
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